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Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation

Oncogene volume 25, pages 5113–5124 (2006)Cite this article

Abstract

We have previously shown that interferon regulatory factor-2 (IRF-2) is acetylated in a cell growth-dependent manner, which enables it to contribute to the transcription of cell growth-regulated promoters. To clarify the function of acetylation of IRF-2, we investigated the proteins that associate with acetylated IRF-2. In 293T cells, the transfection of p300/CBP-associated factor (PCAF) enhanced the acetylation of IRF-2. In cells transfected with both IRF-2 and PCAF, IRF-2 associated with endogenous nucleolin, while in contrast, minimal association was observed when IRF-2 was transfected with a PCAF histone acetyl transferase (HAT) deletion mutant. In a pull-down experiment using stable transfectants, acetylation-defective mutant IRF-2 (IRF-2K75R) recruited nucleolin to a much lesser extent than wild-type IRF-2, suggesting that nucleolin preferentially associates with acetylated IRF-2. Nucleolin in the presence of PCAF enhanced IRF-2-dependent H4 promoter activity in NIH3T3 cells. Nucleolin knock-down using siRNA reduced the IRF-2/PCAF-mediated promoter activity. Chromatin immunoprecipitation analysis indicated that PCAF transfection increased nucleolin binding to IRF-2 bound to the H4 promoter. We conclude that nucleolin is recruited to acetylated IRF-2, thereby contributing to gene regulation crucial for the control of cell growth.

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References

  • Bannister AJ, Miska EA . (2000). Cell Mol life Sci 57: 1184–1192.

  • Barlev N, Liu L, Chehab N, Mansfield K, Harris K, Halazonetis T et al. (2001). Mol Cell 8: 1243–1254.

  • Benkirane M, Chun RF, Xiao H, Ogryzko VV, Howard BH, Nakatani Y et al. (1998). J Biol Chem 273: 24898–24905.

  • Caillaud A, Prakash A, Smith E, Masumi A, Hovanessian A, Levy D et al. (2002). J Biol Chem 277: 49417–49421.

  • Chen L-F, Mu Y, Greene WC . (2002). EMBO J 21: 6539–6548.

  • Chow W, Fang J, Yee J . (2000). J Immunol 164: 3512–3518.

  • Daniely Y, Dimitrova DD, Borowiec JA . (2002). Mol Cell Biol 22: 6014–6022.

  • Deng L, de la Fuente C, Fu P, Wang L, Donnelly R, Wade JD et al. (2000). Virology 277: 278–295.

  • Ginisty H, Sicard H, Roger B, Bouvet P . (1992). J Cell Sci 112: 761–772.

  • Grinstein E, Wernet P, Snijders PJF, Rosl F, Weinert I, Jia W et al. (2002). J Exp Med 196: 1067–1078.

  • Hamamori Y, Sartorelli V, Ogruzko V, Puri PL, Wu HY, Wang JY et al. (1999). Cell 96: 405–413.

  • Harrod R, Kuo YL, Tang Y, Yao Y, Vassilev A, Nakatani Y et al. (2000). J Biol Chem 275: 11852–11857.

  • Hirano M, Kaneko S, Yamashita T, Luo H, Qin W, Shirota Y et al. (2003). J Biol Chem 278: 5109–5115.

  • Jiang H, Lu H, Schiltz RL, Pise-Masison CA, Ogryzko VV, Nakatani Y et al. (1999). Mol Cell Biol 19: 8136–8145.

  • Kiernan RE, Vanhulle C, Schiltz L, Adam E, Xiao H, Maudoux F et al. (1999). EMBO J 18: 6106–6118.

  • Klibanov S, O'Hagen H, Ljungman M . (2001). J Cell Sci 114: 1867–1873.

  • Lakin ND, Jackson SP . (1999). Oncogene 18: 7644–7655.

  • Lang S, Hearing P . (2003). Oncogene 22: 2836–2841.

  • Lapeyre B, Bourbon H, Amalric F . (1987). Proc Natl Acad Sci 84: 1472–1476.

  • Lau OD, Coutney AD, Vassilev A, Marzilli LA, Cotter RJ, Nakatani Y et al. (2000a). J Biol Chem 275: 21953–21959.

  • Lau OD, Kundu TK, Soccio RE, Ait-Si-Ali S, Khalil EM, Vassilev A et al. (2000b). Mol Cell 3: 589–595.

  • Levy L, Wei Y, Labalette C, Wu Y, Renard CA, Buendia MA et al. (2004). Mol Cell Biol 24: 3404–3414.

  • Li J, O'Malley BW, Wong J . (2000). Mol Cell Biol 20: 2031–2042.

  • Luo W, Skalnik DG . (1996). J Biol Chem 271: 23445–23451.

  • Martinez-Balbas MA, Baner UM, Nielsen SJ, Brehm A, Kouzarides T . (2000). EMBO J 19: 662–671.

  • Masumi A, Ozato K . (2001). J Biol Chem 276: 20973–20980.

  • Masumi A, Wang I-M, Lefebvre B, Yang X-J, Nakatani Y, Ozato K . (1999). Mol Cell Biol 19: 1810–1820.

  • Masumi A, Yamakawa Y, Fukazawa H, Ozato K, Komuro K . (2003). J Biol Chem 278: 25401–25407.

  • Ott M, Schnolzer M, Gamica J, Fischle W, Emiliani S, Rackwiz HR et al. (1999). Curr Biol 9: 1489–1492.

  • Patel J, Du Y, Ard P, Phillips C, Carella B, Chen C et al. (2004). Mol Cell Biol 24: 10826–10834.

  • Polwsskaya A, Naguibneva I, Duquet A, Bengal E, Robin P, Harel-Bellan A . (2001). Mol Cell Biol 21: 5312–5320.

  • Santos-Rosa H, Valls1 E, Kouzarides T, Martinez-Balbas M . (2003). Nucl Acids Res 31: 4285–4292.

  • Schaffer BC, Paulson E, Strominger JL, Speck SH . (1997). Mol Cell Biol 17: 873–886.

  • Schiltz RL, Mizzen CA, Vassilev A, Cook RG, Allis CD, Nakatani Y . (1999). J Biol Chem 274: 1189–1192.

  • Spilianakis C, Papamatheakis J, Kretsovail A . (2000). Mol Cell Biol 20: 8489–8498.

  • Srivastava M, Pollard HB . (1999). FASEB J 13: 1911–1922.

  • Stellacci E, Testa U, Retrucci E, Benedetti E, Orsatti R, Feccia T et al. (2004). Biochem J 377: 367–378.

  • Sterner DE, Berger SL . (2000). Mol Cell Biol 64: 435–459.

  • Suhara W, Yoneyama M, Kitabayashi I, Fujita T . (2002). J Biol Chem 277: 22304–22313.

  • Taniguchi T, Ogasawara K, Takaoka A, Tanaka N . (2001). Annu Rev Immunol 19: 623–655.

  • Trievel RC, Li FY, Mamorstein R . (2000). Anal Biochem 287: 319–328.

  • Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati ML, Qin J et al. (1998). Mol Cell 2: 869–875.

  • Vaughan PS, van der Meijden CM, Aziz F, Harada H, Taniguchi T, van WA et al. (1998). J Biol Chem 273: 194–199.

  • Vo N, Goodman RH . (2001). J Biol Chem 276: 13505–13508.

  • Wang I-M, Blanco JCG, Tsai SY, Tsai M-J, Ozato K . (1996). Mol Cell Biol 16: 6313–6324.

  • Wolf D, Rodova M, Miska EA, Calvet JP, Kouzarides T . (2002). J Biol Chem 28: 25562–25567.

  • Xie R, van Wijnen AJ, van der Meijden C, Luong MX, Stein JL, Stein GS . (2001). J BIol Chem 276: 18624–18632.

  • Yamamoto H, Lamphier M, Fujita T, Taniguchi T, Harada H . (1994). Oncogene 9: 1423–1428.

  • Yamauchi T, Yamauchi J, Kuwata T, Tamura T, Yamashita T, Bae N et al. (2000). Proc Natl Acad Sci USA 97: 11303–11306.

  • Yanagida M, Shimamoto A, Nishikawa K, Furuichi Y, Takahashi N . (2001). Proteomics 1: 1390–1404.

  • Ying G-G, Proost P, van Damme J, Bruschi M, Introna M, Golay J . (2000). J Biol Chem 275: 4152–4158.

  • Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E, Fujita T . (1998). EMBO J 17: 1087–1095.

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Acknowledgements

This work was supported by the Japan Society for Promotion of Sciences and the Ministry of Education, Science, Sports and Culture and the Japan Health Sciences International Foundation. We thank Dr Y Nakatani for providing plasmids, Dr K Sakai and Dr M Kasai for technical advices, Dr K Kamemura, Dr A Ito, Dr Y Murakami and Dr I Hamaguchi for useful discussions, and Dr A Fuse and Dr Y Uehera for general support.

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Authors and Affiliations

  1. Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan

    A Masumi, K Komuro & K Yamaguchi

  2. Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan

    H Fukazawa

  3. Chemical Genetics Laboratory, RIKEN, Saitama, Japan

    T Shimazu & M Yoshida

  4. Laboratory of Molecular Growth and Regulation, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD, USA

    K Ozato

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  1. A Masumi
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Correspondence to A Masumi.

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Masumi, A., Fukazawa, H., Shimazu, T. et al. Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation. Oncogene 25, 5113–5124 (2006). https://doi.org/10.1038/sj.onc.1209522

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