Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Human TDE1, a TDE1/TMS family member, inhibits apoptosis in vitro and stimulates in vivo tumorigenesis

Oncogene volume 25, pages 4549–4558 (2006)Cite this article

Abstract

We have previously described hTDE1, the human homologue of the recently described TDE1/TMS family of proteins whose members have been identified in several species. Although a defined biochemical activity has yet to be assigned to TDE1/TMS family members, previous results point to the overexpression of family members in tumor cell lines or tissues. To define whether hTDE1 may directly impact on neoplastic transformation, we derived and characterized stable Rat-1 transfectants that constitutively express hTDE1 at the plasma membrane. Expression of hTDE1 in Rat-1 transfectants had a significant effect on cell contact inhibition in vitro as judged by a focus formation assay. In addition, by monitoring caspase-3 activity and Hoechst staining, we determined that hTDE1 overexpression partially protects cells from serum starvation- and etoposide-induced apoptosis. Finally, hTDE1 Rat-1-expressing clones accelerated the formation of tumors in a nude mouse assay. Our results suggest that hTDE1 contributes directly to oncogenesis in vivo that may in part be explained by its effect on apoptosis in vitro.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  • Aoki S, Su Q, Li H, Nishikawa K, Ayukawa K, Hara Y et al. (2002). J Neurosci 22: 10751–10760.

  • Bazzoni G, Dejana E . (2004). Physiol Rev 84: 869–901.

  • Bossolasco M, Lebel M, Lemieux N, Mes-Masson AM . (1999). Mol Carcinogen 26: 189–200.

  • Chalifour LE, Gomes ML, Wang NS, Mes-Masson AM . (1990). Oncogene 5: 1719–1726.

  • Chalifour LE, Mes-Masson AM, Gomes ML, Wang NS . (1992). Mol Carcinogen 5: 178–189.

  • Corn PG, El-Deiry WS . (2002). BioEssays 24: 83–90.

  • Faust M, Montenarh M . (2000). Cell Tissue Res 301: 329–340.

  • Ferrigno P, Silver PA . (1999). Methods Cell Biol 58: 107–122.

  • Grazia Lampugnani M, Zanetti A, Corada M, Takahashi T, Balconi G, Breviario F et al. (2003). J Cell Biol 161: 793–804.

  • Green DR, Evan GI . (2002). Cancer Cell 1: 19–30.

  • Grossman TR, Luque JM, Nelson N . (2000). J Exp Biol 203 (Part 3): 447–457.

  • Inuzuka M, Hayakawa M, Ingi I . (2005). J Biol Chem 280: 35776–35783.

  • Krueger WH, Gonye GE, Madison DL, Murray KE, Kumar M, Spoerel N et al. (1997). J Neurochem 69: 1343–1355.

  • Kufe DW, Pollock RE, Weichselbaum RR, Bast Jr RC, Gansler TS, Holland JF et al. (eds). (2003). Cancer Medicine, 6th edn. BC Decker Inc.: Hamilton (Canada).

    Google Scholar 

  • Lebel M, Mes-Masson AM . (1994). DNA Sequence 5: 31–39.

  • Nowell PC . (2002). Semin Cancer Biol 12: 261–266.

  • Ormerod MG, Collins MK, Rodriguez-Tarduchy G, Robertson D . (1992). J Immunol Methods 153: 57–65.

  • Pearce DA . (2000). J Neurosci Res 59: 19–23.

  • Player A, Gillespie J, Fujii T, Fukuoka J, Dracheva T, Meerzaman D et al. (2003). Int J Cancer 107: 238–243.

  • Rassoulzadegan M, Naghashfar Z, Cowie A, Carr A, Grisoni M, Kamen R et al. (1983). Proc Natl Acad Sci USA 80: 4354–4358.

  • Renan MJ . (1993). Mol Carcinogen 7: 139–146.

  • Ronot X, Doisy A, Tracqui P . (2000). Cytometry 41: 19–30.

  • Royle SJ, Murrell-Lagnado RD . (2003). BioEssays 25: 39–46.

  • Shigeta M, Sanzen N, Ozawa M, Gu J, Hasegawa H, Sekiguchi K . (2003). J Cell Biol 163: 165–176.

  • Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S . (2000). EMBO Rep 1: 287–292.

  • Stevens FJ, Argon Y . (1999). Semin Cell Dev Biol 10: 443–454.

  • Temin HM, Rubin H . (1958). Virology 6: 669–688.

  • Xu J, Ji C, Wang L, Cao Y, Dai J, Ye X et al. (2003). Mol Biol Rep 30: 47–52.

Download references

Acknowledgements

We thank Jason Madore for technical assistance, and Wissal El-Assaad and Abdellah Belmaaza for stimulating discussions. This research was funded in part by a Canadian Institute of Health Research (CIHR) grant and a Cancer Research Society grant to A-MM-M MB has been supported by a CIHR studentship as well as awards from the Canderel and Marc-Bourgie funds of the Institut du cancer de Montréal. FV was the recipient of a Canderel studentship award. A-MM-M is the recipient of a Fonds de la recherche en santé au Québec (FRSQ) Chercheur National Fellowship. RB is the recipient of an FRSQ Senior Fellowship.

Author information

Authors and Affiliations

  1. Centre de recherche du Centre Hospitalier de l'Université de Montréal (CR-CHUM) and Institut du cancer de Montréal, Montreal, Quebec, Canada

    M Bossolasco, F Veillette, R Bertrand & A-M Mes-Masson

  2. Department of Medicine, Université de Montréal, Montreal, Quebec, Canada

    R Bertrand & A-M Mes-Masson

Authors
  1. M Bossolasco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F Veillette
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R Bertrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A-M Mes-Masson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A-M Mes-Masson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bossolasco, M., Veillette, F., Bertrand, R. et al. Human TDE1, a TDE1/TMS family member, inhibits apoptosis in vitro and stimulates in vivo tumorigenesis. Oncogene 25, 4549–4558 (2006). https://doi.org/10.1038/sj.onc.1209488

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1209488

Keywords

This article is cited by

Search

Advanced search

Quick links