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A functionally distinct member of the DP family of E2F subunits

Oncogene volume 25, pages 3212–3218 (2006)Cite this article

Abstract

E2F transcription factors regulate genes involved in cell-cycle progression. In mammalian cells, physiological E2F exists as an E2F/DP heterodimer. Currently, eight E2F and two DP subunits have been characterized. We report here the characterization of a new member of the DP family, DP-4. While DP-4 exhibits certain similarities with members of the DP family, it also possesses a number of significant differences. Thus, DP-4 forms a heterodimer with E2F subunits, binds to the E2F site and associates with pocket proteins including pRb. In contrast to DP-1, however, DP-4/E2F-1 complexes exhibit reduced DNA binding activity. Furthermore, DP-4 interferes with E2F-1-dependent transcription and delays cell-cycle progression. These results highlight an emerging complexity in the DP family of E2F subunits, and suggest that DP-4 may endow E2F heterodimers with distinct transcription properties.

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Acknowledgements

We thank Rosemary Williams for help in preparing the manuscript, and the AICR, EC, MRC and CRUK for supporting the work.

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Authors and Affiliations

  1. Nuffield Department of Clinical Laboratory Sciences, Medical Sciences Division, Laboratory of Cancer Biology, University of Oxford, John Radcliffe Hospital, Oxford, Oxon, UK

    A Milton, K Luoto, L Ingram, S Munro, N Logan, A L Graham & N B La Thangue

  2. The Netherlands Cancer Institute, Plesmanlaan, Amsterdam, The Netherlands

    T R Brummelkamp, E M Hijmans & R Bernards

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  1. A Milton
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  2. K Luoto
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  3. L Ingram
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  4. S Munro
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  5. N Logan
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  6. A L Graham
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  7. T R Brummelkamp
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  8. E M Hijmans
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  9. R Bernards
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  10. N B La Thangue
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Correspondence to N B La Thangue.

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Milton, A., Luoto, K., Ingram, L. et al. A functionally distinct member of the DP family of E2F subunits. Oncogene 25, 3212–3218 (2006). https://doi.org/10.1038/sj.onc.1209343

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