Abstract
Ligand-induced membrane trafficking of the anaplastic lymphoma kinase (ALK) was studied using a chimeric receptor in which the extracellular and transmembrane domain of ALK was substituted for the corresponding regions of epidermal growth factor receptor (EGFR). Wild-type EGFR, EGFR/ALK and an EGFR/ALK kinase negative mutant were independently expressed in mouse NR6 fibroblasts. The capacity of EGFR/ALK to mediate [125I]-EGF internalization, receptor degradation and downregulation, which has never been previously described, was assayed. The rate of [125I]-EGF-induced internalization mediated by the cytoplasmic domain of ALK was reduced several fold compared with the wild-type EGFR. The low rate of EGF internalization promoted by EGFR/ALK correlated with an impaired degradation and downregulation of the receptor and indicate that ALK is not subject to traditional mechanisms used to regulate receptor tyrosine kinase function. Accordingly, ALK-activated intracellular domain does not associate in vivo with c-cbl and does not undergo ligand-mediated ubiquitination. The current study provides new insight into the function and regulation of ALK suggesting that the relative long membrane residence of activated ALK might confers a more potent and prolonged signaling activity. Indeed NR6-EGFR/ALK cells exhibited a ∼3-fold increase in a maximal mitogenic response than NR6-EGFR.
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Abbreviations
- ALCL:
-
anaplastic large cell lymphoma
- ALK:
-
anaplastic lymphoma kinase
- EGF:
-
epidermal growth factor
- EGFR:
-
epidermal growth factor receptor
- PTyr:
-
phosphotyrosine
- RTK:
-
receptor tyrosine kinase
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Acknowledgements
We are grateful to Professor A Rappelli for his continues support and encouragement during the course of this work. We also thank Professor PP Pelicci and Professor B Falini for providing us with the anti-alk monoclonal antibody. The authors appreciate the excellent assistance of Dr R Bacchiocchi, D Carbonari and M Cesaroni. This research was fully supported by grant from the Associazione Italiana Ricerca sul Cancro. MS is supported by a Fellowship from Fondazione Italiana Ricerca Cancro.
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Serresi, M., Piccinini, G., Pierpaoli, E. et al. A ligand-inducible anaplastic lymphoma kinase chimera is endocytosis impaired. Oncogene 23, 1098–1108 (2004). https://doi.org/10.1038/sj.onc.1207227
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DOI: https://doi.org/10.1038/sj.onc.1207227
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