1. ¹Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique CNRS UMR 8501, Bât. 503, Université Paris XI, 91403 Orsay, France
2. ²CREAB, UMR 5819 (CEA, CNRS, UJF), Département de Recherche Fondamentale sur la Matière Condensée, 17 rue des Martyrs, 38054 Grenoble, France
3. ³Laboratoire de génotoxicologie des tumeurs, EA 3493 UPMC-IC, Hôpital tenon, Pneumologie, 4 rue de la Chine, 75019 Paris, France

Correspondence: T Soussi, E-mail: thierry.soussi@tnn.ap-hop-paris.fr

⁴These authors contributed equally to this study

Received 11 November 2003; Revised 2 February 2004; Accepted 17 February 2004; Published online 7 June 2004.

Abstract

The greatest challenge in the postgenomic era is the description of proteome interactions, such as protein–protein or protein–DNA interactions. Surface plasmon resonance (SPR) is an optical technique in which binding of an analyte to the surface changes the refractive index at the surface/solution interface. Molecular interactions are analysed in real time without a labeling step. Currently, the limit to SPR imaging is the small number of reactions that can be simultaneously analysed. Using a novel grafting technology and a new imaging system, we increased the throughput of SPR imaging. The interaction between p53 and DNA was chosen as a paradigm for validation of this assay. Using a tagged DNA methodology, we simultaneously targeted multiple DNA sequences on a single chip. The interaction between p53 and these DNA sequences was monitored by SPR imaging. Qualitative and quantitative analysis provides results similar to those obtained with conventional technologies.