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Versatile analysis of multiple macromolecular interactions by SPR imaging: application to p53 and DNA interaction

Oncogene volume 23, pages 5543–5550 (2004)Cite this article

Abstract

The greatest challenge in the postgenomic era is the description of proteome interactions, such as protein–protein or protein–DNA interactions. Surface plasmon resonance (SPR) is an optical technique in which binding of an analyte to the surface changes the refractive index at the surface/solution interface. Molecular interactions are analysed in real time without a labeling step. Currently, the limit to SPR imaging is the small number of reactions that can be simultaneously analysed. Using a novel grafting technology and a new imaging system, we increased the throughput of SPR imaging. The interaction between p53 and DNA was chosen as a paradigm for validation of this assay. Using a tagged DNA methodology, we simultaneously targeted multiple DNA sequences on a single chip. The interaction between p53 and these DNA sequences was monitored by SPR imaging. Qualitative and quantitative analysis provides results similar to those obtained with conventional technologies.

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References

  • Balagurumoorthy P, Sakamoto H, Lewis MS, Zambrano N, Clore GM, Gronenborn AM, Appella E and Harrington RE . (1995). Proc. Natl. Acad. Sci. USA, 92, 8591–8595.

  • Bergamaschi D, Gasco M, Hiller L, Sullivan A, Syed N, Trigiante G, Yulug I, Merlano M, Numico G, Comino A, Attard M, Reelfs O, Gusterson B, Bell AK, Heath V, Tavassoli M, Farrell PJ, Smith P, Lu X and Crook T . (2003). Cancer Cell, 3, 387–402.

  • Deppert W, Gohler T, Koga H and Kim E . (2000). J. Cell. Biochem. Suppl., (Suppl 35), 115–122.

  • Dong SM, Traverso G, Johnson C, Geng L, Favis R, Boynton K, Hibi K, Goodman SN, D’Allessio M, Paty P, Hamilton SR, Sidransky D, Barany F, Levin B, Shuber A, Kinzler KW, Vogelstein B and Jen J . (2001). J. Natl. Cancer Inst., 93, 858–865.

  • Drewes G and Bouwmeester T . (2003). Curr. Opin. Cell. Biol., 15, 199–205.

  • El-Deiry WS, Kern SE, Pientenpol JA, Kinzler KW and Vogelstein B . (1992). Nat. Genet., 1, 45–49.

  • Favis R, Day JP, Gerry NP, Phelan C, Narod S and Barany F . (2000). Nat. Biotechnol., 18, 561–564.

  • Fried M and Crothers DM . (1981). Nucleic Acids Res., 9, 6505–6525.

  • Friedlander P, Haupt Y, Prives C and Oren M . (1996). Mol. Cell. Biol., 16, 4961–4971.

  • Galas DJ and Schmitz A . (1978). Nucleic Acids Res., 5, 3157–3170.

  • Gerry NP, Witowski NE, Day J, Hammer RP, Barany G and Barany F . (1999). J. Mol. Biol., 292, 251–262.

  • Guedon P, Livache T, Martin F, Lesbre F, Roget A, Bidan G and Levy Y . (2000). Anal. Chem., 72, 6003–6009.

  • Halazonetis TD, Davis LJ and Kandil AN . (1993). EMBO J., 12, 1021–1028.

  • Hansen S, Hupp TR and Lane DP . (1996). J. Biol. Chem., 271, 3917–3924.

  • Inga A, Storici F, Darden TA and Resnick MA . (2002). Mol. Cell. Biol., 22, 8612–8625.

  • Irwin MS, Kondo K, Marin MC, Cheng LS, Hahn WC and Kaelin WG . (2003). Cancer Cell, 3, 403–410.

  • Kato S, Han SY, Liu W, Otsuka K, Shibata H, Kanamaru R and Ishioka C . (2003). Proc. Natl. Acad. Sci. USA, 100, 8424–8429.

  • Kim E and Deppert W . (2003). Biochem. Cell. Biol., 81, 141–150.

  • Livache T, Maillart E, Lassalle N, Mailley P, Corso B, Guedon P, Roget A and Levy Y . (2003). J. Pharm. Biomed. Anal., 32, 687–696.

  • Melino G, De Laurenzi V and Vousden KH . (2002). Nat. Rev. Cancer, 2, 605–615.

  • Muller BF, Paulsen D and Deppert W . (1996). Oncogene, 12, 1941–1952.

  • Qian H, Wang T, Naumovski L, Lopez CD and Brachmann RK . (2002). Oncogene, 21, 7901–7911.

  • Resnick MA and Inga A . (2003). Proc. Natl. Acad. Sci. USA, 100, 9934–9939.

  • Rich RL and Myszka DG . (2000). Curr. Opin. Biotechnol., 11, 54–61.

  • Rowan S, Ludwig RL, Haupt Y, Bates S, Lu X, Oren M and Vousden KH . (1996). EMBO J., 15, 827–838.

  • Samuels-Lev Y, O’Connor DJ, Bergamaschi D, Trigiante G, Hsieh JK, Zhong S, Campargue I, Naumovski L, Crook T and Lu X . (2001). Mol. Cell, 8, 781–794.

  • Soussi T and Béroud C . (2001). Nat. Rev. Cancer, 1, 233–240.

  • Soussi T and Béroud C . (2003). Hum. Mutat., 21, 192–200.

  • Tokino T, Thiagalingam S, El -Deiry WS, Waldman T, Kinzler KW and Vogelstein B . (1994). Hum. Mol. Gen., 3, 1357–1542.

  • Vogelstein B, Lane D and Levine AJ . (2000). Nature, 408, 307–310.

  • Vousden KH and Lu X . (2002). Nat. Rev. Cancer, 2, 594–604.

  • Walhout AJ and Vidal M . (2001). Nat. Rev. Mol. Cell. Biol., 2, 55–62.

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Acknowledgements

This work was supported by grants from the Association de la Recherche sur le Cancer (TS No. 4809), Ligue Nationale Contre le Cancer (Comité de Paris), the European Community's Fifth Framework Programme (QLRT-1999-00273), and Université PM Curie to TS. DC was supported by a fellowship from the Association de la Recherche sur le Cancer.

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Author notes

  1. Emmanuel Maillart and Karen Brengel-Pesce: These authors contributed equally to this study

Authors and Affiliations

  1. Laboratoire Charles Fabry de l’Institut d’Optique (LCFIO), Centre National de la Recherche Scientifique CNRS UMR 8501, Bât. 503, Université Paris XI, Orsay, 91403, France

    Emmanuel Maillart, Michael Canva & Yves Levy

  2. Département de Recherche Fondamentale sur la Matière Condensée, CREAB, UMR 5819 (CEA, CNRS, UJF), 17 rue des Martyrs, Grenoble, 38054, France

    Karen Brengel-Pesce, André Roget & Thierry Livache

  3. Laboratoire de génotoxicologie des tumeurs, EA 3493 UPMC-IC, Hôpital tenon, Pneumologie, 4 rue de la Chine, Paris, 75019, France

    Delphine Capela & Thierry Soussi

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  1. Emmanuel Maillart
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  2. Karen Brengel-Pesce
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Correspondence to Thierry Soussi.

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Supplementary Information accompanies the paper on Oncogene website (http://www.nature.com/onc)

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Maillart, E., Brengel-Pesce, K., Capela, D. et al. Versatile analysis of multiple macromolecular interactions by SPR imaging: application to p53 and DNA interaction. Oncogene 23, 5543–5550 (2004). https://doi.org/10.1038/sj.onc.1207639

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