Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Transactivation mediated by B-Myb is dependent on TAFII250

Oncogene volume 22, pages 2932–2941 (2003)Cite this article

Abstract

B-Myb is a highly conserved member of the Myb family of transcription factors, which has been implicated in cell cycle regulation. B-Myb is expressed in most proliferating cells and its activity is highly regulated around the G1/S-phase border of the cell cycle. It is generally assumed that B-Myb regulates the expression of genes that are crucial for cell proliferation; however, the identity of these genes, the molecular mechanisms by which B-Myb stimulates their expression and the involvement of other proteins have not been sufficiently clarified. We have employed the hamster cell line ts13 as a tool to demonstrate a functional link between B-Myb and the coactivator TAFII250, a key component of the transcriptional machinery which itself is essential for cell proliferation. ts13 cells express a point-mutated version of TAFII250 whose intrinsic histone acetyl transferase activity is temperature sensitive. Transactivation of Myb-responsive reporter genes by B-Myb is temperature-dependent in ts13 cells but not in ts13 cells, which have been rescued by transfection with an expression vector for wild-type TAFII250. Furthermore, B-Myb and TAFII250 can be coprecipitated, suggesting that both proteins are present in a complex. The formation of this complex is dependent on the DNA-binding domain of B-Myb and not on its transactivation domain. Taken together, these observations provide the first evidence that the coactivator TAFII250 is involved in the activation of Myb responsive promoters by B-Myb. The finding that B-Myb transactivation is dependent on a key coactivator involved in cell cycle control is consistent with and strengthens the idea that B-Myb plays a crucial role as a transcription factor in proliferating cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6

Similar content being viewed by others

References

  • Albright SR and Tjian R . (2000). Gene, 242, 1–13.

  • Arsura M, Introna M, Passerini F, Mantovani A and Golay J . (1992). Blood, 79, 2708–2716.

  • Bartsch O, Horstmann S, Toprak K, Klempnauer K-H and Ferrari S . (1999). Eur. J. Biochem., 260, 384–391.

  • Bessa M, Saville MK and Watson RJ . (2001). Oncogene, 20, 3376–3386.

  • Bouwmeester T, Guehmann S, el-Baradi T, Kalkbrenner F, van Wijk I, Moelling K and Pieler T . (1992). Mech. Dev., 37, 57–68.

  • Burk O, Mink S, Ringwald M and Klempnauer KH . (1993). EMBO J., 12, 2027–2038.

  • Burk O, Worpenberg S, Haenig B and Klempnauer K-H . (1997). EMBO J., 16, 1371–1380.

  • Cervellera MN and Sala A . (2000). Poly(ADP-ribose) polymerase is a B-Myb coactivator. J. Biol. Chem., 275, 10692–10696.

    Article  CAS  Google Scholar 

  • Charrasse S, Carena I, Brondani V, Klempnauer KH and Ferrari S . (2000). Oncogene, 19, 2986–2995.

  • Dikstein R, Ruppert S and abd Tjian R . (1996). Cell, 84, 781–790.

  • Dunphy EL, Johnson T, Auerbach SS and Wang EH . (2000). Mol. Cell. Biol., 20, 1134–1139.

  • Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB and Livingston DM . (1994). Genes Dev., 8, 869–884.

  • Foos G, Grimm S and Klempnauer KH . (1992). EMBO J., 11, 4619–4629.

  • Gill G, Pascal E, Tseng H and Tjian R . (1994). Proc. Natl. Acad. Sci. USA, 91, 192–196.

  • Hisatake K, Hasegawa S, Takada R, Nakatani Y, Horikoshi M and Roeder RG . (1993). Nature, 362, 179–181.

  • Horstmann S, Ferrari S and Klempnauer KH . (2000a). Oncogene, 19, 5428–5434.

  • Horstmann S, Ferrari S and Klempnauer KH . (2000b). Oncogene, 19, 298–306.

  • Imhof A, Yang XJ, Ogryzko VV, Nakatani Y, Wolffe AP and Ge H . (1997). Curr. Biol., 7, 689–692.

  • Johnson LR, Johnson TK, Desler M, Luster TA, Nowling T, Lewis RE, Rizzino A . (2002). J. Biol. Chem., 277, 4088–4097.

  • Kamano H, Burk B, Noben-Trauth K and Klempnauer KH . (1995). Oncogene, 11, 2575–2582.

  • Kamano H and Klempnauer KH . (1997). Oncogene, 14, 1223–1229.

  • Lam EW, Robinson C and Watson RJ . (1992). Oncogene, 7, 1885–1890.

  • Lam EW and Watson RJ . (1993). EMBO J., 12, 2705–2713.

  • Lane S, Farlie P and Watson R . (1997). Oncogene, 14, 2445–2453.

  • Levaillard T and Wasylyk B . (1997). J. Biol. Chem., 272, 30651–30661.

  • Li X and McDonnell DP . (2002) Mol. Cell. Biol., 22, 3663–3673.

  • Lin D, Fiscella M, O'Connor PM, Jackman J, Chen M, Luo LL, Sala A, Travali S, Appella E and Mercer WE . (1994). Proc. Natl. Acad. Sci. USA, 91, 10079–10083.

  • Lively TN, Ferguson HA, Galasinski SK, Seto AG and Goodrich JA . (2001). J. Biol. Chem., 276, 25582–25588.

  • Mink S, Kerber U and Klempnauer K-H . (1996). Mol. Cell. Biol. 16, 1316–1325.

  • Mizzen CA, Yang X-J, Kokubo T, Brownell JF, Bannister AJ, Owen-Hughes T, Workman J, Wang L, Berger SL, Kouzarides T, Nakatani Y and Allis CD . (1996). Cell, 87, 1261–1270.

  • Moscovici C, Moscovici MG, Jiminez H, Lai MMC, Hayman MJ and Vogt PK . (1977). Cell, 11, 95–103.

  • Nomura N, Takahashi M, Matsui M, Ishii S, Date T, Sasamoto S and Ishizaki R . (1988). Nucleic Acids Res., 16, 11075–11089.

  • O'Brien T and Tjian R . (1998). Mol. Cell., 1, 905–911.

  • O'Brien T and Tjian R . (2000). Proc. Natl. Acad. Sci. USA., 97, 2456–2461.

  • Pham A-D and Sauer F . (2000). Science, 289, 2357–2360.

  • Robinson C, Light Y, Groves R, Mann D, Marais R and Watson R . (1996). Oncogene, 12, 1855–1864.

  • Sala A, Calabretta B (1992). Proc. Natl. Acad. Sci. USA, 89, 10415–10419.

  • Sala A, Kundu M, Casella I, Engelhard A, Calabretta B, Grasso L, Paggi MG, Giordano A, Watson RJ, Khalili K and Peschle C . (1997). Proc. Natl. Acad. Sci. USA, 94, 532–536.

  • Saville MK and Watson RJ . (1998). Oncogene, 17, 2679–2689.

  • Siegert JL and Robbins PD . (1999). Mol. Cell. Biol., 19, 846–854.

  • Siegert JL, Rushton JJ, Seller WR, Kaelin WG and Robbins P . (2000). Oncogene, 19, 5703–5711.

  • Sitzmann J, Noben-Trauth K, Kamano H and Klempnauer KH . (1996). Oncogene, 12, 1889–1894.

  • Suzuki-Yagawa Y, Guermah M and Roeder RG . (1997). Mol. Cell. Biol., 17, 3284–3294.

  • Tahirov TH, Sato K, Ichikawa-Iwata E, Sasaki M, Inoue-Bungo T, Shiina M, Kimura, K, Takata S, Fujikawa A, Morii H, Kumasaka T, Yamamoto M, Ishii S and Ogata K . (2002). Cell, 108, 57–70.

  • Talavera A and Basilico C . (1977). J. Cell. Physiol., 92, 425–436.

  • Wang EH and Tjian R . (1994). Science, 263, 811–814.

  • Wang EH, Zou S and Tjian R . (1997). Genes Dev., 11, 2658–2669.

  • Wassarman DA and Sauer F . (2001). J. Cell Sci., 114, 2895–2902.

  • Weissman JD, Brown JA, Howcroft TK, Hwang J, Chawla A, Roche PA, Schiltz L, Nakatani Y and Singer DS . (1998). Proc. Natl. Acad. Sci. USA, 95, 11601–11606.

  • Yang SH, Vickers E, Brehm A, Kouzarides T and Sharrocks AD . (2001). Mol. Cell. Biol., 21, 2802–2814.

  • Ziebold U, Bartsch O, Marais R, Ferrari S and Klempnauer KH . (1997). Curr. Biol., 7, 253–260.

  • Ziebold U and Klempnauer KH . (1997). Oncogene, 15, 1011–1019.

  • Zwicker J, Liu N, Engeland K, Lucibello FC and Muller R . (1996). Science, 271, 1595–1597.

Download references

Acknowledgements

We thank S Horstmann for valuable discussions, EH Wang for providing cell lines and plasmids and for critical comments on this manuscript and A Brehmer-Fastnacht for excellent technical assistance. This study was supported by a grant from the DFG (KL 461/9-2) and by the Fonds der chemischen Industrie.

Author information

Authors and Affiliations

  1. Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, Münster, D-48149, Germany

    Thorsten Bartusel & Karl-Heinz Klempnauer

Authors
  1. Thorsten Bartusel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karl-Heinz Klempnauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karl-Heinz Klempnauer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bartusel, T., Klempnauer, KH. Transactivation mediated by B-Myb is dependent on TAFII250. Oncogene 22, 2932–2941 (2003). https://doi.org/10.1038/sj.onc.1206494

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206494

Keywords

This article is cited by

Search

Advanced search

Quick links