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  • Oncogenomics
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Identification of radiation-specific responses from gene expression profile

Oncogene volume 21, pages 8521–8528 (2002)Cite this article

Abstract

The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in γ-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make ‘RadChip’. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon γ-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cγ2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after γ-radiation in RT–PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-κB upon γ-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.

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Acknowledgements

We thank Dr Dong-Sup Lee for his helpful discussions and our laboratory members for their helpful inspiration to this study. J-H Kim and J-H Woo are elective students for this project (2001). This work was supported by Nuclear Research Funds from KISTEP to W-Y Park (1999–2001). This study was supported in part by 2001 BK21 project for Medicine, Dentistry and Pharmacy to M-J Kang, C-N Im, C-I Hwang, W-Y Park and J-S Seo.

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Authors and Affiliations

  1. Ilchun Molecular Medicine Institute, Seoul National University, Seoul, Korea

    Woong-Yang Park & Jeong-Sun Seo

  2. Department of Biochemistry and Molecular Biology, Seoul National University, Seoul, Korea

    Woong-Yang Park, Chang-Il Hwang, Chang-Nim Im, Min-Ji Kang, Jang-Hee Woo, Ju-Hoon Kim & Jeong-Sun Seo

  3. Department of Internal Medicine, Seoul National University, Seoul, Korea

    Yon Su Kim

  4. Department of Preventive Medicine, College of Medicine, Seoul National University, Seoul, Korea

    Ju-Han Kim

  5. Department of Biostatistics, Graduate School of Public Health, Seoul National University, Seoul, Korea

    Ho Kim

  6. Macrogen Inc., Seoul, Korea

    Kyung-A Kim & Hyung-Jin Yu

  7. Laboratory of Radiation Effect, Korea Cancer Center Hospital, Seoul, Korea

    Sue-Jae Lee & Yun-Sil Lee

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  1. Woong-Yang Park
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Correspondence to Jeong-Sun Seo.

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Park, WY., Hwang, CI., Im, CN. et al. Identification of radiation-specific responses from gene expression profile. Oncogene 21, 8521–8528 (2002). https://doi.org/10.1038/sj.onc.1205977

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