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Deregulation of FCGR2B expression by 1q21 rearrangements in follicular lymphomas

Oncogene volume 20, pages 7686–7693 (2001)Cite this article

Abstract

We report here the molecular cloning and characterization of a t(1;14)(q21;q32) in a follicular lymphoma (FL) with an unusual BCL2 aberration. Fluorescence in situ hybridization (FISH) and Southern blot analysis of tumor cells identified the translocation breakpoint within the 5′ switch region of IGHG (Sγ). We cloned the chimeric breakpoint region approximately 1.5 kbp downstream from the HindIII site of 5′Sγ2 on chromosome 14q32 and identified a 360-bp novel segment with homology to the CpG island clone 11h8. Two BAC clones containing this sequence were isolated and mapped to 1q21 by FISH. BAC 342/P13 contained sequences homologous to Fcγ receptors 2A, 3A, 2B, 3B, and a heat shock protein gene HSP70B. The translocation brought the Sγ2 region of a productive IGH allele 20∼30 kbp upstream of FCGR2B. As a result of the translocation, the b2 isoform of FCGR2B was overexpressed in the tumor. Screening of a panel of 76 B-cell lymphomas with 1q21-23 cytogenetic aberrations by Southern blot analysis using breakpoint probes identified an additional FL with a t(14;18)(q32;q21) and a breakpoint in the FCGR2B region. These results suggest that FCGR2B may be deregulated by 1q21 aberration in BCL2 rearranged FLs and possibly play a role in their progression.

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References

  • Bergsagel PL, Chesi M, Nardini E, Brents LA, Kirby SL, Kuehl WM . 1996 Proc. Natl. Acad. Sci. USA 93: 13931–13936

  • Bolland S, Pearse RN, Kurosaki T, Ravetch JV . 1998 Immunity 8: 509–516

  • Brooks DG, Qiu WQ, Luster AD, Ravetch JV . 1989 J. Exp. Med. 170: 1369–1385

  • Budde P, Bewarder N, Weinrich V, Schulzeck O, Frey J . 1994 Biol. Chem. 269: 30636–30644

  • Callanan MB, Le Baccon P, Mossuz P, Duley S, Bastard C, Hamoudi R, Dyer MJ, Klobeck G, Rimokh R, Sotto JJ, Leroux D . 2000 Proc. Natl. Acad. Sci. USA 97: 309–314

  • Chaganti RSK, Nanjangud G, Schmidt H, Teruya-Feldstein J . 2000 Semin. Hematol. 37: 396–411

  • Chaganti SR, Rao PH, Chen W, Dyomin V, Jhanwar SC, Parsa NZ, Dalla-Favera R, Chaganti RSK . 1998 Genes Chromosomes Cancer 23: 328–336

  • Cigudosa JC, Parsa NZ, Louie DC, Filippa DA, Jhanwar SC, Johansson B, Mitelman F, Chaganti RSK . 1999 Genes Chromosomes Cancer 25: 123–133

  • Daeron M . 1997 Annu. Rev. Immunol. 15: 203–234

  • Dalla-Favera R, Migliazza A, Chang CC, Niu H, Pasqualucci L, Butler M, Shen Q, Cattoretti G . 1999 Curr. Top. Microbiol. Immunol. 246: 257–263

  • Dyomin VG, Palanisamy N, Lloyd KO, Dyomina K, Jhanwar SC, Houldsworth J, Chaganti RSK . 2000 Blood 95: 2666–2671

  • Hatzivassiliou G, Miller I, Takizawa J, Palanisamy N, Rao PH, Iida S, Tagawa S, Taniwaki M, Russo J, Neri A, Cattoretti G, Clynes R, Mendelsohn C, Chaganti RSK, Dalla-Favera R . 2001 Immunity 14: 277–289

  • Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA . 1998 Blood 91: 2369–2380

  • Offit K, Wong G, Filippe DA, Tao Y, Chaganti RSK . 1991 Blood 77: 1508–1515

  • Ono M, Bolland S, Tempst P, Ravetch JV . 1996 Nature 383: 263–266

  • Pearse RN, Kawabe T, Bolland S, Guinamard R, Kurosaki T, Ravetch JV . 1999 Immunity 10: 753–760

  • Qiu WQ, de Bruin D, Brownstein BH, Pearse R, Ravetch JV . 1990 Science 248: 732–735

  • Rao PH, Murty VV, Gaidano G, Hauptschein R, Dalla-Favera R, Chaganti RSK . 1993 Genomics 16: 426–430

  • Ravetch JV, Lanier LL . 2000 Science 290: 84–89

  • Sarmay G, Rozsnyay Z, Koncz G, Danilkovich A, Gergely J . 1995 Eur. J. Immunol. 25: 262–268

  • Tamir I, Stolpa JC, Helgason CD, Nakamura K, Bruhns P, Daeron M, Cambier JC . 2000 Immunity 12: 347–358

  • Tilly H, Rossi A, Stamatoullas A, Lenormand B, Bigorgne C, Kunlin A, Monconduit M, Bastard C . 1994 Blood 84: 1043–1049

  • Tweeddale ME, Lim B, Jamal N, Robinson J, Zalcberg J, Lockwood G, Minden MD, Messner HA . 1987 Blood 69: 1307–1314

  • Willis TG, Zalcberg IR, Coignet LJ, Wlodarska I, Stul M, Jadayel DM, Bastard C, Treleaven JG, Catovsky D, Silva ML, Dyer MJ . 1998 Blood 91: 1873–1881

  • Willis TG, Dyer MJ . 2000 Blood 96: 808–822

  • Witz IP, Rhan M . 1992 Immunol. Res. 11: 283–295

  • Yamanishi Y, Tamura T, Kanamori T, Yamane H, Nariuchi H, Yamamoto T, Baltimore D . 2000 Genes Dev. 14: 11–16

  • Zusman T, Lisansky E, Arons E, Bonnerot C, Sautes C, Fridman WH, Witz IP, Ran M . 1996 Int. J. Cancer 68: 219–227

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Acknowledgements

We thank Jeffrey Ravetch for his interest in this work and for discussions about the function of FCR genes. Supported by the National Institutes of Health-National Cancer Institute grants CA-34775, CA-66999 and CA-80814 (RSK Chaganti).

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Authors and Affiliations

  1. Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA

    Weiyi Chen, Nallasivam Palanisamy, Helmut Schmidt, Jane Houldsworth & R S K Chaganti

  2. Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA

    Julie Teruya-Feldstein

  3. Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York, USA

    Suresh C Jhanwar, Andrew D Zelenetz & R S K Chaganti

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  1. Weiyi Chen
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  2. Nallasivam Palanisamy
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Correspondence to R S K Chaganti.

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Chen, W., Palanisamy, N., Schmidt, H. et al. Deregulation of FCGR2B expression by 1q21 rearrangements in follicular lymphomas. Oncogene 20, 7686–7693 (2001). https://doi.org/10.1038/sj.onc.1204989

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