Abstract
Rel/NF-κB transcription factors control a variety of cellular processes, such as cell growth and apoptosis, that are relevant to oncogenesis, and mutations in genes encoding Rel/NF-κB transcription factors have been found in several human lymphoid cell cancers. In this study, we have used a sensitive cell outgrowth assay to demonstrate that wild-type human c-Rel can malignantly transform primary chicken spleen cells, and that transformation by c-Rel is accelerated by co-expression of Bc1-2. Full-length mouse c-Rel can also transform chicken spleen cells. These results are the first demonstration of a lymphoid cell malignant transforming ability for mammalian Rel/NF-κB transcription factors, and implicate c-Rel as a molecular target for cancer therapeutics.
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Acknowledgements
We thank N Rice for human c-Rel antiserum, P Schreiber-Schwartz and DJ Waxman for Bc1-2 and Bc1-xL antisera, D Kalaitzidis for creating the JD214BS+ retroviral vector for the expression of human c-rel and for helpful discussions, and G Cooper for comments on the manuscript. This work was supported by NIH grant CA47763 (to TD Gilmore). C Cormier performed research as part of the Honors Research Program in Biochemistry & Molecular Biology, and was supported by funds from the Beckman Foundation and from the Undergraduate Research Opportunities Program of Boston University.
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Gilmore, T., Cormier, C., Jean-Jacques, J. et al. Malignant transformation of primary chicken spleen cells by human transcription factor c-Rel. Oncogene 20, 7098–7103 (2001). https://doi.org/10.1038/sj.onc.1204898
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DOI: https://doi.org/10.1038/sj.onc.1204898
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