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Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130

Oncogene volume 20, pages 5533–5537 (2001)Cite this article

Abstract

The murine Unp gene encodes a widely expressed ubiquitin-specific protease. The predicted sequence of the UNP protein features motifs common to viral oncoproteins through which these proteins interact with the retinoblastoma gene product pRb, as well as the related ‘pocket proteins’ p107 and p130. We have explored the possibility that UNP interacts with pocket proteins, and report here that such associations can be detected in vitro and in cells. Associations of UNP and pocket proteins are sensitive to site-directed mutations in a manner directly analogous to those documented in viral oncoproteins. We conclude that within cells UNP does physically associate with pRb, and can also associate with p107 and p130.

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Acknowledgements

This work was supported by National Cancer Institute of Canada grant number 010068, awarded to DA Gray, and by a studentship from the National Science and Engineering Research Council of Canada to P Blanchette. We are indebted to Dr Michael Rudnicki for the gift of lysates from p107 and p130 null mice.

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  1. Catherine A Gilchrist

    Present address: Department of Biological Sciences, Stanford University, Stanford, 94305-5020, CA, USA

Authors and Affiliations

  1. Centre for Cancer Therapeutics, Ottawa Regional Cancer Centre, 503 Smyth Road, Ottawa, K1H 1C4, Canada

    Paola Blanchette & Douglas A Gray

  2. Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, GPO Box 334 (Mills Road), Canberra, ACT 2601, Australia

    Catherine A Gilchrist & Rohan T Baker

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  1. Paola Blanchette
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  2. Catherine A Gilchrist
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Blanchette, P., Gilchrist, C., Baker, R. et al. Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130. Oncogene 20, 5533–5537 (2001). https://doi.org/10.1038/sj.onc.1204823

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