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Cloning and characterization of a novel transcription factor involved in cellular proliferation arrest: PATF

Oncogene volume 20, pages 5475–5483 (2001)Cite this article

Abstract

Cell cycle withdrawal involves several transcription factors such as E2Fs members that play a key role in cell growth control. Here we describe a novel putative bZIP transcription factor isolated from the retina and involved in neuronal proliferation arrest at the terminal differentiation: PATF (Proliferation Arrest Transcription Factor). We show that PATF associates with E2F4 protein and interacts with the E2F consensus site. PATF expression increases with establishment of quiescent state. Furthermore, the nuclear PATF localization like E2F4, depends on cell growth arrest. The decrease of PATF amount, using a retroviral antisense strategy, results in pursued neuroretina cell mitosis. Our results indicate that PATF could be a new molecular signal implicated in the final neuronal cell cycle withdrawal.

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References

  • Bidou L, Crisanti P, Blancher C, Pessac B . 1993 Mech. Dev. 43: 159–173

  • Biedler JL, Helson L, Spengler BA . 1973 Cancer Res. 33: 2643–2652

  • Bradford MM . 1976 Anal. Biochem. 72: 248–254

  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ . 1979 Biochemistry 18: 5294–5299

  • Crisanti P, Lorinet AM, Calothy G, Pessac B . 1985 EMBO J. 4: 1467–1470

  • Crisanti-Combes P, Lorinet AM, Girard A, Pessac B, Wassef M, Calothy G . 1982 Cell Differ. 11: 45–54

  • Crisanti-Combes P, Privat A, Pessac B, Calothy G . 1977 Cell Tissue Res. 185: 159–173

  • Dyer MA, Cepko CL . 2000 Development 127: 3593–3605

  • Espanel X, Le Cam L, North S, Sardet C, Brun G, Gillet G . 1998 Oncogene 17: 585–594

  • He X, Rosenfeld MG . 1991 Neuron 7: 183–196

  • Helin K . 1998 Curr. Opin. Genet. Dev. 8: 28–35

  • Hughes SH, Greenhouse JJ, Petropoulos CJ, Sutrave P . 1987 J. Virol. 61: 3004–3012

  • Kastner A, Espanel X, Brun G . 1998 Cell Growth Differ. 9: 857–867

  • Leone G, Nuckolls F, Ishida S, Adams M, Sears R, Jakoi L, Miron A, Nevins JR . 2000 Mol. Cell Biol. 20: 3626–3632

  • Levine EM, Close J, Fero M, Ostrovsky A, Reh TA . 2000 Dev. Biol. 219: 299–314

  • Maniatis T, Fritsch EF, Shambrook J . (1989) Molecular Cloning: A laboratory manual 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Nevins JR . 1998 Cell Growth Differ. 9: 585–593

  • Olson EN . 1990 Genes Dev. 4: 1454–1461

  • Omri B, Blancher C, Neron B, Marty MC, Rutin J, Molina TJ, Pessac B, Crisanti P . 1998 Oncogene 16: 2351–2356

  • Persengiev SP, Kardova II, Kilpatrick DL . 1999 Mol. Cell. Biol. 19: 6048–6056

  • Reid TW, Albert DM, Rabson AS, Russell P, Craft J, Chu EW, Tralka TS, Wilcox JL . 1974 J. Natl. Cancer Inst. 53: 347–360

  • Rouet P, Raguenez G, Tronche F, Yaniv M, N'Guyen C, Salier JP . 1992 J. Biol. Chem. 267: 20765–20773

  • Slansky JE, Farnham PJ . 1996 Curr. Top. Microbiol. Immunol. 208: 1–30

  • Struhl K . 1991 Neuron 7: 177–181

  • Trimarchi JM, Fairchild B, Verona R, Moberg K, Andon N, Lees JA . 1998 Proc. Natl. Acad. Sci. USA 95: 2850–2855

  • Vigo E, Muller H, Prosperini E, Hateboer G, Cartwright P, Moroni MC, Helin K . 1999 Mol. Cell Biol. 19: 6379–6395

  • Wang SZ, Adler R . 1994 Proc. Natl. Acad. Sci. USA 91: 1351–1355

  • Wu CL, Zukerberg LR, Ngwu C, Harlow E, Lees JA . 1995 Mol. Cell Biol. 15: 2536–2546

  • Yamasaki L . 1998 Results Probl. Cell Differ. 22: 199–227

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Acknowledgements

This work was supported by INSERM and grants from Association de la Recherche contre le Cancer (ARC), Association Retina France (AFRP) and Association Claude Bernard. We thank Dr Mishal Zohar for FACS and Confocal Microscopy analyses, CNRS, IFC1 Laboratoire de Cytométrie–Villejuif and Christophe Klein for confocal microscopy, Institut Biomedical des Cordeliers IFR 58.

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Authors and Affiliations

  1. Développement, Vieillissement et Pathologie de la Rétine, INSERM U450, Affiliée CNRS, Association Claude Bernard, 29 rue Wilhem, Paris, 75016, France

    Patricia Crisanti, Gilda Raguenez, Christine Blancher, Bertrand Neron, Asmaa Mamoune & Boubaker Omri

  2. Université Paris VI, France

    Boubaker Omri

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  1. Patricia Crisanti
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  2. Gilda Raguenez
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  3. Christine Blancher
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Correspondence to Patricia Crisanti.

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Crisanti, P., Raguenez, G., Blancher, C. et al. Cloning and characterization of a novel transcription factor involved in cellular proliferation arrest: PATF. Oncogene 20, 5475–5483 (2001). https://doi.org/10.1038/sj.onc.1204711

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