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Cloning and characterization of ectopically expressed transcripts for the actin-binding protein MIPP in mouse mammary carcinomas

Oncogene volume 20, pages 5366–5372 (2001)Cite this article

Abstract

Mipp is a kelch-related, placental-specific gene that is ectopically expressed in many BALB/c mouse mammary carcinomas of various etiologies. The Kelch family encompasses proteins that are emerging as key links between microfilaments and a variety of cellular structures and functions. Mouse mammary tumors express two mipp transcripts (2.2 and 5.6 kb). We cloned the 2.2 kb mipp mRNA and analysed the product of its 1.7 kb ORF. The 584 residue MIPP protein has an N-terminal BTB domain and six C-terminal tandem Kelch repeats. Despite expression of two mipp RNAs, only a single MIPP protein is expressed in mammary tumors. MIPP protein binds to microfilaments in vitro and co-immunoprecipitates with actin. MIPP co-localized with concanavalin A at the endoplasmic reticulum, suggesting that MIPP might mediate interactions between microtubules and actin filaments. Because MIPP expression is widespread in mouse mammary tumors, it might contribute to tumorigenesis. Although MIPP had little effect on the growth rate of human breast cell lines following transfection, it greatly reduced the formation of duct-like structures on reconstituted basement membrane. Our results suggest that MIPP could contribute to malignant progression in the mouse mammary epithelial cells by perverting their response to cues from the extracellular matrix.

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References

  • Albagli O, Dhordain P, Deweindt C, Lecocq G, Leprince D . 1995 Cell Growth Differ 6: 1193–1198

  • Bork P, Doolittle RF . 1994 J. Mol. Biol. 236: 1277–1282

  • Callebaut I, Mornon JP . 1998 Cell. Mol. Life Sci. 54: 880–891

  • Cardiff RD, Aguilar-Cordova E . 1988 Anticancer Res. 8: 925–933

  • Carraway KL, Carraway CAC . 1992 The Cytoskeleton: a practical approach IRL Press at Oxford University, New York

    Google Scholar 

  • Chang-Yeh A, Mold DE, Brilliant MH, Huang RC . 1993 Proc. Natl. Acad. Sci. USA 90: 292–296

  • Chang-Yeh A, Mold DE, Huang RC . 1991 Nucleic Acids Res. 19: 3667–3672

  • Eichinger L, Bomblies L, Vandekerckhove J, Schleicher M, Gettemans J . 1996 EMBO J. 15: 5547–5556

  • Goode BL, Drubin DG, Barnes G . 2000 Curr. Opin. Cell. Biol. 12: 63–71

  • Gould MN . 1995 Semin. Cancer Biol. 6: 147–152

  • Gregory RB, Wilcox RA, Berven LA, van Straten NC, van der Marel GA, van Boom JH, Barritt GJ . 1999 Biochem. J. 341: 401–408

  • Hernandez MC, Andres-Barquin PJ, Martinez S, Bulfone A, Rubenstein JL, Israel MA . 1997 J. Neurosci. 17: 3038–3051

  • Horton P, Nakai K . 1997 ISMB 5: 147–152

  • Kim IF, Mohammadi E, Huang RCC . 1999 Gene. 228: 73–83

  • Laemmli UK . 1970 Nature 227: 680–685

  • Lueders KK, Kuff EL . 1977 Cell 12: 963–972

  • Mata J, Nurse P . 1997 Cell 89: 939–949

  • Medina D . 1988 Carcinogenesis 9: 1113–1119

  • Medina D, Daniel C . 1996 J. Mammary Gland Biol. Neoplasia 1: 3–4

  • Medina D, Kittrell FS . 1993 Carcinogenesis 14: 25–28

  • Medina D, Kittrell FS, Liu YJ, Schwartz M . 1993 Cancer Res. 53: 663–667

  • Prinz WA, Grzyb L, Veenhuis M, Kahana JA, Silver PA, Rapoport TA . 2000 J. Cell Biol. 150: 461–474

  • Ribeiro CM, Reece J, Putney Jr JW . 1997 J. Biol. Chem. 272: 26555–26561

  • Robinson DN, Cooley L . 1997 J. Cell Biol. 138: 799–810

  • Sanders MC, Way M, Sakai J, Matsudaira P . 1996 J. Biol. Chem. 271: 2651–2657

  • Schmid MF, Agris JM, Jakana J, Matsudaira P, Chiu W . 1994 J. Cell Biol. 124: 341–350

  • Schmucker B, Ballhausen WG, Kressel M . 1997 Eur. J. Cell. Biol. 72: 46–53

  • Selden SC, Pollard TD . 1986 Ann. NY Acad. Sci. 466: 803–812

  • Soltysik-Espanola M, Rogers RA, Jiang S, Kim TA, Gaedigk R, White RA, Avraham H, Avraham S . 1999 Mol. Biol. Cell. 10: 2361–2375

  • Tabb JS, Molyneaux BJ, Cohen DL, Kuznetsov SA, Langford GM . 1998 J. Cell. Sci. 111: 3221–3234

  • Terasaki M . 1990 Cell. Motil Cytoskeleton 15: 71–75

  • VanHouten JN, Natoli F, Asch BB . 1996 Oncogene 12: 2241–2245

  • Waterman-Storer CM, Salmon E . 1999 Curr. Opin. Cell. Biol. 11: 61–67

  • Wood JG, Byrd FI, Gurd JW . 1981 J. Neurocytol 10: 149–159

  • Xue F, Cooley L . 1993 Cell. 72: 681–693

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Acknowledgements

We thank Dr Linda Varela for her gift of EHS matrix. This work was supported by Predoctoral Fellowship DAMD 17-96-1-6048 from the Department of the Army's Breast Cancer Research Program and by grants CA62014, CA72768, and Roswell Park Cancer Institute's CCSG CA16056 from the NCI, NIH.

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  1. Joshua N VanHouten

    Present address: Division of Endocrinology and Metabolism, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, 06520, USA

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  1. Division of Experimental Pathology, Roswell Park Cancer Institute Corporation, New York, Buffalo, NY 14263, USA

    Joshua N VanHouten, Harold L Asch & Bonnie B Asch

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  1. Joshua N VanHouten
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Correspondence to Joshua N VanHouten.

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VanHouten, J., Asch, H. & Asch, B. Cloning and characterization of ectopically expressed transcripts for the actin-binding protein MIPP in mouse mammary carcinomas. Oncogene 20, 5366–5372 (2001). https://doi.org/10.1038/sj.onc.1204701

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