Abstract
We report the identification of a novel human tumor associated gene, CDCP1 (Cub Domain Containing Protein), which was identified using representational difference analysis and cDNA chip technology. The gene consists of eight exons, the upstream region of which neither contains a TATA- nor a CCAAT-box. However, a CpG island is located around the transcription start, which is found in approximately 60% of known genes. The CDCP1 gene was mapped to chromosome 3p21-p23 by fluorescence in situ hybridization. For expression profiling real time quantitative RT–PCR was performed using cell lines and laser capture microdissected colon cancer biopsies. CDCP1 mRNA is approximately 6 kb and highly overexpressed in human colon cancer and lung cancer. CDCP1 represents a putative transmembrane protein, containing three CUB domains in the extracellular part most likely involved in cell adhesion or interacting with the extracellular matrix.
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References
Abe K . 1992 Mamm. Genome 2: 252–259
Afshari CA, Nuwaysir EF, Barrett JC . 1999 Cancer Res. 59: 4759–4760
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ . 1997 Nucleic Acids Res. 25: 3389–3402
Bateman A, Birney E, Durbin R, Eddy SR, Howe KL, Sonnhammer EL . 2000 Nucleic Acids Res. 28: 263–266
Bhatia-Gaur R, Donjacour AA, Sciavolino PJ, Kim M, Desai N, Young P, Norton CR, Gridley T, Cardiff RD, Cunha GR, Abate-Shen C, Shen MM . 1999 Genes Dev. 13: 966–977
Bird AP . 1986 Nature 321: 209–213
Bork P, Beckmann G . 1993 J. Mol. Biol. 231: 539–545
Bornstein SR, Willenberg HS, Scherbaum WA . 1998 Med. Klin. 93: 739–743
Brendel V, Bucher P, Nourbakhsh IR, Blaisdell BE, Karlin S . 1992 Proc. Natl. Acad. Sci. USA 89: 2002–2006
Brown PO, Botstein D . 1999 Nat. Genet. 21: 33–37
Bucher P . 1990 J. Mol. Biol. 212: 563–578
Cangi MG, Cukor B, Soung P, Signoretti S, Moreira Jr G, Ranashinge M, Cady B, Pagano M, Loda M . 2000 J. Clin. Invest 106: 753–761
Cross SH, Clark VH, Simmen MW, Bickmore WA, Maroon H, Langford CF, Carter NP, Bird AP . 2000 Mamm. Genome 11: 373–383
Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD . 1996 Proc. Natl. Acad. Sci. USA 93: 6025–6030
Fearon ER, Vogelstein B . 1990 Cell 61: 759–767
Fialkow PJ . 1976 Biochim. Biophys. Acta 458: 283–321
Gerstein M, Jansen R . 2000 Curr. Opin. Struct. Biol. 10: 574–584
Hanahan D, Weinberg RA . 2000 Cell 100: 57–70
Hubank M, Schatz DG . 1994 Nucleic Acids Res. 22: 5640–5648
Hubank M, Schatz DG . 1999 Meth. Enzymol. 303: 325–349
Ishii M, Hashimoto S, Tsutsumi S, Wada Y, Matsushima K, Kodama T, Aburatani H . 2000 Genomics 68: 136–143
King RD, Sternberg MJ . 1996 Protein Sci. 5: 2298–2310
Knight SJ, Horsley SW, Regan R, Lawrie NM, Maher EJ, Cardy DL, Flint J, Kearney L . 1997 Eur. J. Hum. Genet. 5: 1–8
Kozak M . 1987 Nucleic Acids Res. 15: 8125–8148
Kurian KM, Watson CJ, Wyllie AH . 1999 J. Pathol. 187: 267–271
Liang P, Pardee AB . 1992 Science 257: 967–971
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC . 1988 Hum. Genet. 80: 224–234
Mitelman F, Mertens F, Johansson B . 1997 Nat. Genet. 15: 417–474
Nielsen H, Engelbrecht J, Brunak S, von Heijne G . 1997 Protein Eng 10: 1–6
Nowell PC . 1976 Science 194: 23–28
Parle-McDermott A, McWilliam P, Tighe O, Dunican D, Croke DT . 2000 Br. J. Cancer 83: 725–728
Romero A, Romao MJ, Varela PF, Kolln I, Dias JM, Carvalho AL, Sanz L, Topfer-Petersen E, Calvete JJ . 1997 Nat. Struct. Biol. 4: 783–788
Sambrook J, Fritsch EF, Maniatis T . 1989 Molecular Cloning: A laboratory Manual 2nd ed Cold Spring Harbor, Laboratory Press, Cold Spring Harbor, NY, USA
Schultz J, Copley RR, Doerks T, Ponting CP, Bork P . 2000 Nucleic Acids Res. 28: 231–234
Sidransky D, Mikkelsen T, Schwechheimer K, Rosenblum ML, Cavanee W, Vogelstein B . 1992 Nature 355: 846–847
Sonnhammer EL, von Heijne G, Krogh A . 1998 Ismb. 6: 175–182
Stollberg J, Urschitz J, Urban Z, Boyd CD . 2000 Genome Res. 10: 1241–1248
To KY . 2000 Comb. Chem. High Throughput. Screen. 3: 235–241
Tusnady GE, Simon I . 1998 J. Mol. Biol. 283: 489–506
Velculescu VE, Zhang L, Vogelstein B, Kinzler KW . 1995 Science 270: 484–487
Wickner WT, Lodish HF . 1985 Science 230: 400–407
Wootton JC, Federhen S . 1996 Meth. Enzymol. 266: 554–571
Acknowledgements
We thank H Dauwa-Stummer and A Gupta for excellent technical assistance and R Varecka for DNA sequencing. We would like to acknowledge K Zatloukal (Dept. of Pathology, University of Graz) for generously providing the colon cancer patient samples and we want to thank L Wightman for critically reading the manuscript. Last but not least we want to thank KK Wilgenbus, G Adolf and M Baccarini for helpful discussions.
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Scherl-Mostageer, M., Sommergruber, W., Abseher, R. et al. Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene 20, 4402–4408 (2001). https://doi.org/10.1038/sj.onc.1204566
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DOI: https://doi.org/10.1038/sj.onc.1204566
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