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The chicken Pdcd4 gene is regulated by v-Myb

Oncogene volume 20, pages 231–239 (2001)Cite this article

Abstract

The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the ability of avian myeloblastosis virus (AMV) to transform myelomonocytic cells. v-Myb is thought to disrupt the differentiation of myelomonocytic cells by affecting the expression of specific target genes. To identify such genes we have analysed the gene expression in a myelomonocytic chicken cell line that carries an estrogen inducible version of v-Myb by differential display. Here we describe the identification of the chicken homolog of the mouse Pdcd4 gene as a novel v-Myb target gene. Pdcd4 is also known as MA-3, TIS and H731 and has recently been shown to suppress the transformation of epidermal cells by tumor promoters. Our results provide the first evidence that v-Myb directly regulates the expression of a potential tumor suppressor gene.

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Acknowledgements

We thank A Brehmer-Fastnacht for excellent technical assistance and J-M Buerstedde and T Graf for providing some of the cells used in this study. This work was supported by grants from the SFB364/A3 and by the Fonds der Chemischen Industrie.

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Author notes

  1. Oliver Burk

    Present address: Dr Margarete Fischer-Bosch-Institut für klinische Pharmakologie, Auerbachstr. 112, Stuttgart, D-70376

  2. Susanne Worpenberg

    Present address: Max-Planck-Institute for Immunobiology, Stübeweg 51, Freiburg, D-79108, Germany

Authors and Affiliations

  1. Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, Münster, D-48149, Germany

    Ursula Schlichter, Oliver Burk, Susanne Worpenberg & Karl-Heinz Klempnauer

  2. Germany

    Oliver Burk

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Schlichter, U., Burk, O., Worpenberg, S. et al. The chicken Pdcd4 gene is regulated by v-Myb. Oncogene 20, 231–239 (2001). https://doi.org/10.1038/sj.onc.1204071

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