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  • Original Paper
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NO activation of fos promoter elements requires nuclear translocation of G-kinase I and CREB phosphorylation but is independent of MAP kinase activation

Oncogene volume 19, pages 6324–6333 (2000)Cite this article

Abstract

We have shown that nitric oxide (NO) regulates c-fos gene expression via cGMP-dependent protein kinase (G-kinase), but NO's precise mechanism of action is unclear. We now demonstrate that: (1) NO targets two transcriptional elements in the fos promoter, i.e., the fos AP-1 binding site and the cAMP-response element (CRE); (2) NO activation of these two enhancer elements requires the CRE binding protein CREB because a dominant negative CREB fully inhibits NO transactivation of reporter genes whereas dominant negative Fos or CCAAT enhancer binding proteins have no effect; (3) CREB is phosphorylated by G-kinase in vitro and its phosphorylation increases in vivo when G-kinase is activated either directly by cGMP or indirectly by NO via soluble guanylate cyclase; (4) NO activation of fos promoter elements requires nuclear translocation of G-kinase but not activation of mitogen-activated protein kinases.

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Acknowledgements

We would like to thank Drs V Sharma for helpful discussions, S Lohmann for the wild type G-kinase I vector and a rabbit polyclonal anti-G-kinase I antibody, N Nakane for the guanylate cyclase vectors, R Prywes for fos promoter constructs, A Rothman for the CS-54 cells, S Taylor for the purified catalytic domain of A-kinase and J Feramisco and N Varki for help with fluorescence microscopy. This work was supported in part by NIH Grant RO1GM055586 to RB Pilz and American Heart Association Grant 9650582N to GR Boss; T Gudi was supported by NIH grant 5T32DK07233-24.

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Authors and Affiliations

  1. Department of Medicine and Cancer Center, University of California, San Diego, La Jolla, CA 92093-0652, California, USA

    Tanima Gudi, Darren E Casteel, Gerry R Boss & Renate B Pilz

  2. Laboratory of Metabolism, NCI, National Institutes of Health, Bethesda, MD 20892, Maryland, USA

    Charles Vinson

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Gudi, T., Casteel, D., Vinson, C. et al. NO activation of fos promoter elements requires nuclear translocation of G-kinase I and CREB phosphorylation but is independent of MAP kinase activation. Oncogene 19, 6324–6333 (2000). https://doi.org/10.1038/sj.onc.1204007

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