Abstract
By immunoscreening with an antibody raised against a plasma membrane protein, we have cloned a growth suppressor gene, Gros1 and assigned it to short arm of human chromosome 1. Two alternatively spliced forms of the gene encoding 84- and 41-kDa (carboxy-terminus truncated) proteins were cloned. The two transcripts, 4.4 and 2.7 kb, were expressed weakly in most of the human tissues, with a high expression of the smaller transcript in placenta, ovary and testis. Normal human fibroblasts in culture showed two transcripts, with a higher level of expression of the 4.4 kb transcript. Transformed cells on the other hand showed predominant expression of the 2.7 kb transcript. Two Gros1 transcripts were also detected in most of the mouse tissues. Stable transfection of the mouse cDNA encoding the 85-kDa protein into NIH3T3 cells resulted in their slow growth and reduced colony-forming efficiency. Stable clones expressing antisense RNA on the other hand exhibited higher colony forming efficiency. While our data implied that Gros1 is a novel growth suppressor gene on human chromosome 1, an independent study has recently characterized its rat-homolog as a leucine proline-enriched novel basement membrane-associated proteoglycan leprecan. We describe here cloning, expression and biological activity analysis implying that this novel proteoglycan is a potential growth suppressor on chromosome 1p31, frequently altered in many malignancies.
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References
Anttonen A, Kajanti M, Heikkilä P, Jalkanen M and Joensuu H. . 1999 Br. J. Cancer 79: 558–564.
Ariyama T, Inazawa J, Ezaki T, Nakamura Y, Horii A and Abe T. . 1995 Genomics 25: 114–123.
Arlt MF, Herzog TJ, Mutch DG, Gersell DJ, Liu H and Goodfellow PJ. . 1996 Hum. Mol. Genet. 5: 1017–1021.
Bomme L, Heim S, Bardi G, Fenger C, Kronborg O, Brogger A and Lothe RA. . 1998 Genes Chromo. Cancer 21: 185–194.
Borg A, Zhang QX, Olsson H and Wenngren E. . 1992 Genes Chromo. Cancer 5: 311–320.
Caput D. . 1997 Bull. Cancer 84: 1081–1082.
Caron H. . 1995 Med. Pediatr. Oncol. 24: 215–221.
Carrino DA, Sorrell JM and Caplan AI. . 2000 Arch. Biochem. Biophys. 373: 91–101.
Chen HL, Chen YC and Chen DS. . 1996 Cancer Genet. Cytogenet 86: 102–106.
Cheng NC, Van Roy N, Chan A, Beitsma M, Westerveld A, Speleman F and Versteeg R. . 1995 Oncogene 10: 291–297.
Dhodapkar MV, Abe E, Theus A, Lacy M, Langford JK, Barlogie B and Sanderson RD. . 1998 Blood 91: 2679–2688.
Dhodapkar MV and Sanderson RD. . 1999 Leuk. Lymph. 34: 35–43.
Di Vinci A, Infusini E, Nigro S, Monaco R and Giaretti W. . 1998 Cancer 83: 415–422.
Fujimoto Y and Kohgo Y. . 1998 Jp. J. Clinical Pathol. 46: 9–14.
Hocking AM, Shinomura T and McQuillan DJ. . 1998 Matrix Biol. 17: 1–19.
Iolascon A, Faienza MF, Coppola B, Moretti A, Basso G, Amaru R, Vigano G and Biondi A. . 1997 Leukemia 11: 359–363.
Iozzo RV, Moscatello DK, McQuillan DJ and Eichstetter I. . 1999 J. Biol. Chem. 274: 4489–4492.
Ishino S, Hashimoto N, Fushiki S, Date K, Mori T, Fujimoto M, Nakagawa Y, Ueda S, Abe T and Inazawa J. . 1998 Cancer 83: 360–366.
Jost CA, Marin MC and Kaelin Jr WG. . 1997 Nature 389: 191–194.
Kaghad M, Bonnet H, Yang A, Creancier L, Biscan JC, Valent A, Minty A, Chalon P, Lelias JM, Dumont X, Ferrara P, McKeon F and Caput D. . 1997 Cell 90: 809–819.
Kolettas E and Rosenberger RF. . 1998 Eur. J. Biochem. 254: 266–274.
Lee WC, Balsara B, Liu Z, Jhanwar SC and Testa JR. . 1996 Cancer Res. 56: 4297–4301.
Mai M, Huang H, Reed C, Qian C, Smith JS, Alderete B, Jenkins R, Smith DI and Liu W. . 1998 Genomics 51: 359–363.
Merle B, Durussel L, Delmas PD and Clezardin P. . 1999 J. Cell Biochem. 75: 538–546.
Moley JF, Brother MB, Fong CT, White PS, Baylin SB, Nelkin B, Wells SA and Brodeur GM. . 1992 Cancer Res. 52: 770–774.
Munn KE, Walker RA and Varley JM. . 1995 Oncogene 10: 1653–1657.
Nagai H, Negrini M, Carter SL, Gillum DR, Rosenberg AL, Schwartz GF and Croce CM. . 1995 Cancer Res. 55: 1752–1757.
Nakanishi K, Yoshioka N, Oka K and Hakura A. . 1999 Int. J. Cancer. 80: 527–532.
Nash MA, Loercher AE and Freedman RS. . 1999 Cancer Res. 59: 6192–6196.
Oohira A, Matsui F, Tokita Y, Yamauchi S and Aono S. . 2000 Arch. Biochem. Biophys. 374: 24–34.
Perlman EJ, Valentine MB, Griffin CA and Look AT. . 1996 Genes Chromo. Cancer 16: 15–20.
Praml C, Finke LH, Herfarth C, Schlag P, Schwab M and Amler L. . 1995 Oncogene 11: 1357–1362.
Schwab M, Praml C and Amler LC. . 1996 Genes Chromo. Cancer 16: 211–229.
Stander M, Naumann U, Dumitrescu L, Heneka M, Loschmann P, Gulbins E, Dichgans J and Weller M. . 1998 Gene Ther. 5: 1187–1194.
Stander M, Naumann U, Wick W and Weller M. . 1999 Cell Tissue Res. 296: 221–227.
Tsukamoto K, Ito N, Yoshimoto M, Kasumi F, Akiyama F, Sakamoto G, Nakamura Y and Emi M. . 1998 Cancer 82: 317–322.
Verbeek MM, Otte-Holler I, van den Born J, van den Heuvel LP, David G, Wesseling P and de Waal RM. . 1999 Am. J. Pathol. 155: 2115–2125.
Wadhwa R, Kaul SC, Ikawa Y and Sugimoto Y. . 1991 Mutat. Res. 256: 243–254.
Wadhwa R, Kaul SC, Ikawa Y and Sugimoto Y. . 1993 J. Biol. Chem. 268: 6615–6621.
Wassenhove-McCarthy DJ and McCarthy KJ. . 1999 J. Biol. Chem. 274: 25004–25017.
White PS, Maris JM, Sulman EP, Jensen SJ, Kyemba SM, Beltinger CP, Allen C, Kramer DL, Biegel JA and Brodeur GM. . 1997 Eur. J. Cancer 33: 1957–1961.
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Kaul, S., Sugihara, T., Yoshida, A. et al. Gros1, a potential growth suppressor on chromosome 1: its identity to basement membrane-associated proteoglycan, leprecan. Oncogene 19, 3576–3583 (2000). https://doi.org/10.1038/sj.onc.1203696
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DOI: https://doi.org/10.1038/sj.onc.1203696
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