Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

cRel-TD kinase: a serine/threonine kinase binding in vivo and in vitro c-Rel and phosphorylating its transactivation domain

Oncogene volume 19, pages 2224–2232 (2000)Cite this article

Abstract

The activity of transcription factors is often modulated by signal responsive protein kinases. Rel/NF-κB transcription factors are regulated by IκB inhibitors, the phosphorylation of which causes ubiquitination and degradation, resulting in nuclear translocation of NF-κB and activation of target genes. Here we report pull-down and immunoprecipitation experiments showing that a mammalian 66 kDa protein kinase binds murine c-Rel, both in vitro and in vivo. This kinase appears to have at least two binding sites on c-Rel, a proline-directed serine/threonine substrate specificity similar to MAP kinases and to specifically phosphorylate the C-terminal domain of murine c-Rel at an ERK consensus site.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7

Similar content being viewed by others

References

  • Abe MK, Kuo W-L, Hershenson MB and Rosner MR . 1999 Mol Cell Biol 19: 1301–1312

  • Baichwal VR and Baeuerle PA . 1997 Curr Biol 7: 94–96

  • Baldwin Jr AS . 1996 Annu Rev Immunol 14: 649–681

  • Baniahmad A, Kohne AC and Renkawitz R . 1992 EMBO J 11: 1015–1023

  • Beg AA, Finco TS, Nantermet PV and Baldwin Jr AS . 1993 Mol Cell Biol 13: 3301–3310

  • Berthelsen J, Zappavigna V, Mavilio F and Blasi F . 1998 EMBO J 17: 1423–1433

  • Bird TA, Schooley K, Dower SK, Hagen H and Virca GD . 1997 J Biol Chem 272: 32606–32612

  • Boehmelt G, Madruga J, Dorfler P, Briegel K, Schwartz H, Enrietto PJ and Zenke M . 1995 Cell 80: 341–352

  • Boulton TG, Nye SH, Robbins DJ, Ip NY, Radziejewska E, Morgenbesser SD, DePinho RA, Panayotatos N, Cobb MH and Yancopoulos GD . 1991 Cell 65: 663–675

  • Boyle WJ, Smeal T, Defize LHK, Angel P, Woodgett JR, Karin M and Hunter T . 1991 Cell 64: 573–584

  • Bull P, Morley KL, Hoekstra MF, Hunter T and Verma IM . 1990 Mol Cell Biol 10: 5473–5485

  • Carrasco D, Risso CA, Dorfman K and Bravo R . 1996 EMBO J 15: 3640–3650

  • Chen C, Agnès F and Gélinas C . 1999 Mol Cell Biol 19: 307–316

  • Chen ZJ, Hagler J, Palombella VJ, Melandri F, Scherer D, Ballard D and Maniatis T . 1995 Genes Dev 9: 1586–1597

  • Chen ZJ, Parent L and Maniatis T . 1996 Cell 84: 853–862

  • Cheng M, Boulton TG and Cobb MH . 1996 J Biol Chem 271: 8951–8958

  • Drier EA, Huang LH and Steward R . 1999 Genes Dev 13: 556–568

  • Druker BJ, Neumann M, Okuda K, Franza BRJ and Griffin JD . 1994 J Biol Chem 269: 5387–5390

  • Hansen SK, Baeuerle PA and Blasi F . 1994 Mol Cell Biol 14: 2593–2603

  • Hayashi T, Sekine T and Okamoto T . 1993 J Bio Chem 268: 26790–26795

  • Henkel T, Machleidt T, Alkalay I, Kronke M, Ben-Neriah Y and Baeuerle PA . 1993 Nature 365: 182–185

  • Hunter T and Karin M . 1992 Cell 70: 375–387

  • Jacobs D, Glossip D, Xing H, Muslin AJ and Kornfeld K . 1999 Genes Dev 13: 163–175

  • Kaelin WGJ, Pallas DC, DeCaprio JA, Kaye FJ and Livingston DM . 1991 Cell 64: 521–532

  • Karin M . 1994 Curr Opin Cell Biol 6: 415–424

  • Karin M . 1995 J Biol Chem 270: 16483–16486

  • Kerr LD, Ransone LJ, Wamsley P, Schmitt MJ, Boyer TG, Zhou Q, Berk AJ and Verma IM . 1993 Nature 365: 412–419

  • Knoepfler PS and Kamps MP . 1995 Mol Cell Biol 15: 5811–5819

  • Kopp E and Ghosh S . 1995 Adv Immunol 58: 1–27

  • Kwok RP, Lundblad JR, Chrivia JC, Richards JP, Bachinger HP, Brennan RG, Roberts SG, Green MR and Goodman RH . 1994 Nature 370: 223–226

  • Leppa A, Saffrich R, Ansorge W and Bohmann D . 1998 EMBO J 15: 4404–4413

  • Lewis RB, McClure J, Rup B, Niesel DW, Garry RF, Hoelzer JD, Nazerian K and Bose Jr HR . 1981 Cell 25: 421–431

  • Luque I and Gélina C . 1997 Semin Cancer Biol 8: 103–111

  • Mosialos G, Hamer P, Capobianco AJ, Laursen RA and Gilmore TD . 1991 Mol Cell Biol 11: 5867–5877

  • Naumann M and Scheidereit C . 1994 EMBO J 13: 4597–4607

  • Palombella VJ, Rando OJ, Goldberg AL and Maniatis T . 1994 Cell 78: 773–785

  • Pellicioli A, Lucca C, Liberi G, Marini F, Lopes M, Plevani P, Romano A, Di Fiore PP and Foiani M . 1999 EMBO J 18: 6561–6572

  • Resnati M, Guttinger M, Valcamonica S, Sidenius N, Blasi F and Fazioli F . 1996 EMBO J 15: 1572–1582

  • Rice NR and Ernst MK . 1993 EMBO J 12: 4685–4695

  • Sambrook J, Fritsch EF and Maniatis T . 1989 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

  • Sassone-Corsi P . 1995 Annu Rev Cell Dev Biol 11: 355–377

  • Schmitz ML and Baeuerle PA . 1991 EMBO J 10: 3805–3817

  • Smith DB and Johnson KS . 1988 Gene 67: 31–40

  • Verma IM and Stevenson J . 1997 Proc Natl Acad Sci USA 94: 11758–11760

  • Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D and Miyamoto S . 1995 Genes Dev 9: 2723–2735

  • Xu X, Prorock C, Ishikawa H, Maldonado E, Ito Y and Gélinas C . 1993 Mol Cell Biol 13: 6733–6741

  • Zappavigna V, Sartori D and Mavilio F . 1994 Genes Dev 8: 732–744

  • Zhong H, Su Yang H, Erdjument-Bromage H, Tempst P and Ghosh S . 1997 Cell 89: 413–424

  • Zhong H, Voll R and Ghosh S . 1998 Mol Cell 1: 661–671

Download references

Acknowledgements

The authors wish to thank V Zappavigna (H-S Raffaele, Milan-Italy) for the generous gift of pTUAS plasmid and G Haegemann (Gent, Belgium) for the pABGal4-RelA plasmid. We are also grateful to Simon Plyte (Pharmacia & UpJohn, Milan-Italy) for critical reading of the manuscript and helpful suggestions. We are grateful to PP Di Fiore (E.I.O., Milan-Italy) for stimulating discussion. This work was supported by grants from AIRC (Associazione Italiana per la Ricerca sul Cancro) and EU (contract number BMH4-CT97-2453).

Author information

Authors and Affiliations

  1. Department of Molecular Pathology and Medicine, Molecular Genetics Unit, Università Vita-Salute San Raffaele, Via Olgettina 58, Milan, 20132, Italy

    Caterina Fognani, Rossana Rondi & Francesco Blasi

  2. Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, Milan, 20141, Italy

    Alfredo Romano

Authors
  1. Caterina Fognani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rossana Rondi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alfredo Romano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Francesco Blasi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fognani, C., Rondi, R., Romano, A. et al. cRel-TD kinase: a serine/threonine kinase binding in vivo and in vitro c-Rel and phosphorylating its transactivation domain. Oncogene 19, 2224–2232 (2000). https://doi.org/10.1038/sj.onc.1203543

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1203543

Keywords

This article is cited by

Search

Advanced search

Quick links