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Over-expression of ERT(ESX/ESE-1/ELF3), an ets-related transcription factor, induces endogenous TGF-β type II receptor expression and restores the TGF-β signaling pathway in Hs578t human breast cancer cells

Oncogene volume 19, pages 151–154 (2000)Cite this article

Abstract

The epithelium-specific transcription factor, ERT/ESX/ESE-1/ELF3, binds to the TGF-β RII promoter in a sequence specific manner and regulates its expression. In this study, we investigated whether ERT could regulate endogenous TGF-β RII expression in Hs578t breast cancer cells. Analyses of the Hs578t parental cell line revealed low RII mRNA expression and resistance to the growth inhibitory effects of TGF-β. Infection of this cell line with a retroviral construct expressing ERT induced higher levels of endogenous RII mRNA expression and protein expression relative to cells infected with chloramphenicol acetyltransferase (CATneo) as a control. Relative to control cells, the ERTneo-expressing Hs578t cells show approximately a 50% reduction in cell growth in the presence of exogenous TGF-β1, as well as a fourfold higher induction of activation in transient transfection assays using the 3TP-luciferase reporter construct. When transplanted into athymic mice, ERT-expressing Hs578t cells showed decreased and delayed tumorigenicity compared with control cells. This data strongly suggests that ERT plays an important role as a transcriptional activator of TGF-β RII expression, and that deregulated ERT expression may play a critical role in rendering Hs578t human breast cancer cells insensitive to TGF-β's growth inhibitory effects.

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References

  • Capocasale R, Lamb R, Vonderheid EC, Fox FE, Rook AH, Nowell PC and Moore JS . 1995 Proc Natl Acad Sci USA 92: 5501–5505.

  • Chang C-H, Scott GK, Kuo W-L, Xiong X, Suzdalseva Y, Park JW, Sayre P, Erny K, Collins C, Gray JW and Benz CC . 1997a Oncogene 1: 1617–1622.

  • Chang J, Park K, Bang Y-J, Kim W, Kim D and Kim S-J . 1997b Cancer Res 57: 2856–2859.

  • Choi S-G, Yi Y, Kim Y-S, Kato M, Chang J, Chung H-W, Hahm K-B, Yang H-K, Rhee HH, Bang, Y-J and Kim S-J . 1998 J Biol Chem 273: 110–117.

  • Heldin C-H, Miyazono K and ten Dijke P . 1997 Nature 390: 465–471.

  • Inagaki M, Moustaka A, Lin HY, Lodish HF and Carr BI . 1993 Proc Natl Acad Sci USA 90: 5359–5363.

  • Kadin ME, Cavaille-Coll MW, Gertz R, Massagué J, Cheifetz S and George D . 1994 Proc Natl Acad Sci USA 91: 6002–6006.

  • Kim DH and Kim S-J . 1996 J Biomed Sci 3: 143–158.

  • Kim IY, Ahn H-J, Zelner DJ, Shaw JW, Sensibar JA, Kim J-H, Kato M, and Lee C . 1996 Cancer Res 56: 44–48.

  • Lai Z-C and Rubin GM . 1992 Cell 70: 609–620.

  • Lin H, Wang X-F, Ng-Eaton E, Weinberg R and Lodish H . 1992 Cell 68: 775–785.

  • Macleod K, Leprince D and Stehelin D . 1992 Trends Biochem Sci 17: 251–256.

  • Markowitz SD and Roberts AB . 1996 In Cytokine and Growth Factor Reviews Elsevier London pp 93–102

    Google Scholar 

  • Markowitz S, Wang J, Myeroff L, Parsons R, Sun LZ, Lutterbaugh J, Fan RS, Zborowska E, Kinzler KW, Vogelstein B, Brattain MG and Willson JKV . 1995 Science 268: 1336–1338.

  • Massagué J, Hata A and Liu F . 1997 Trends Cell Biol 7: 187–192.

  • Myeroff LL, Parsons R, Kim S-J, Hedrick L, Cho KR, Orth K, Mathis M, Kinzler KW, Lutterbaugh J, Park K, Bang Y-J, Lee HY, Park JG, Lynch HT, Roberts AB, Vogelstein B and Markowitz SD . 1995 Cancer Res 55: 5545–5547.

  • Oettgen P, Alani RM, Barcinski MA, Brown L, Akbarali Y, Boltax J, Kunsch C, Munger K and Libermann TA . 1997 Mol Cell Biol 17: 4419–4433.

  • Ohashi T, Boggs S, Robbins P, Bahnson A, Patrene K, Wei F-S, Wei J-F, Li J, Lucht L, Fei Y, Clark S, Kimak M, He H, Mowery-Rushton P and Barranger JA . 1992 Proc Natl Acad Sci USA 89: 11332–11336.

  • Park K, Kim S-J, Bang Y-J, Park J-G, Kim NK, Roberts AB and Sporn MB . 1994 Proc Natl Acad Sci USA 91: 8772–8776.

  • Roberts AB and Sporn MB . 1990 In AB Roberts and MB Sporn eds, Peptide Growth Factors and Their Receptors Handbook of Experimental Pharmacology Heidelberg, Springer-Verlag pp 419–472

    Google Scholar 

  • Sgouras DN, Athanasiou MA, Beal Jr GJ, Fisher RJ, Blair DG and Mavrothalassitis GJ . 1995 EMBO J 14: 4781–4793.

  • Sun L, Wu G, Willson JKV, Zborowska E, Yang J, Rajkarunanayake I, Wang J, Gentry LE, Wang X-F and Brattain MG . 1994 J Biol Chem 269: 26449–26455.

  • Tymms MJ, Ng AY, Thomas RS, Schutte BC, Zhou J, Eyre HJ, Sutherland GR, Seth A, Rosenberg M, Papas T, Debouck C and Kola I . 1997 Oncogene 15: 2449–2462.

  • Watson DK, Smyth FE, Thompson DM, Cheng JQ, Testa JR, Papas TS, and Seth A . 1992 Cell Growth Differ 3: 705–713.

  • Wrana JL, Attisano L, Weiser R, Ventura F and Massague J . 1994 Nature 370: 341–347.

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  1. Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, NIH, Bethesda, 20892-5055, Maryland, MD, USA

    Jay Chang, Cecile Lee, Ki-Baik Hahm, Youngsuk Yi, Shin-Geon Choi & Seong-Jin Kim

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  1. Jay Chang
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Chang, J., Lee, C., Hahm, KB. et al. Over-expression of ERT(ESX/ESE-1/ELF3), an ets-related transcription factor, induces endogenous TGF-β type II receptor expression and restores the TGF-β signaling pathway in Hs578t human breast cancer cells. Oncogene 19, 151–154 (2000). https://doi.org/10.1038/sj.onc.1203252

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