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The translation initiation factor, hu-Sui1 may be a target of hepatitis B X antigen in hepatocarcinogenesis

Oncogene volume 18, pages 1677–1687 (1999)Cite this article

Abstract

The role of hepatitis B virus X antigen in the development of hepatocellular carcinoma was explored by stably transfecting HepG2 cells with an X antigen expression vector, and identifying the differences in gene expression that distinguish X positive from X negative cells by subtractive PCR. One differentially expressed gene, the human homolog of sui1 (hu-sui1), encodes a translation initiation factor whose expression was suppressed by X antigen in HepG2 cells. Hu-Sui1 was also expressed in nontumor liver but not in tumor cells from patients with hepatocellular carcinoma. Introduction of hu-sui1 into HepG2 cells inhibited cell growth in culture, in soft agar, and partially inhibited tumor formation in nude mice. Hence, the suppression of hu-sui1 by X antigen may result in the abrogation of negative growth regulation and contribute to the development of hepatocellular carcinoma.

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Acknowledgements

This work was supported by NIH grants CA48656 and CA66971 to MF. EMBL Data Base accession number L26247.

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Authors and Affiliations

  1. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Zhaorui Lian, Jingbo Pan, Jie Liu, ShuMin Zhang & Mark A Feitelson

  2. Department of Medicine, Molecular Hepatology Research Unit, University of the Witwatersrand, Johannesburg, South Africa

    Patrick Arbuthnot & Michael Kew

  3. Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Mark A Feitelson

  4. Department of Pathology, Fourth Military Medical College, Xi'an, Shanxi, PRC

    Minghua Zhu

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Lian, Z., Pan, J., Liu, J. et al. The translation initiation factor, hu-Sui1 may be a target of hepatitis B X antigen in hepatocarcinogenesis. Oncogene 18, 1677–1687 (1999). https://doi.org/10.1038/sj.onc.1202470

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