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GTPase deficient mutant of Gα13 regulates the expression of Egr-1 through the small GTPase Rho

Oncogene volume 18, pages 1639–1642 (1999)Cite this article

Abstract

The α-subunit of the heterotrimeric G protein G13 regulate cell growth, differentiation and apoptosis in different cell types. Expression of the constitutively activated mutant of Gα13 (Gα13QL) increases the expression of Egr-1, an immediate-early response gene that is identified to be involved in cell growth, differentiation, and apoptosis. Here we report that Gα13QL activates the promoter of Egr-1 through specific sequence which includes the characteristic CArG boxes. We also demonstrate that the Gα13QL activation of Egr-1 promoter is mediated by the Ras-like small GTPase Rho.

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Acknowledgements

We are grateful to Dr Vikas P Sukhatme for the promoter constructs of Egr-1 and Dr J Silvio Gutkind for the expression vectors pCEVRacN17. The work was supported by National Institutes of Health Grant GM 49897 (ND).

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  1. Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, 19140, Pennsylvania, USA

    MVVS Vara Prasad & N Dhanasekaran

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  1. MVVS Vara Prasad
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  2. N Dhanasekaran
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Prasad, M., Dhanasekaran, N. GTPase deficient mutant of Gα13 regulates the expression of Egr-1 through the small GTPase Rho. Oncogene 18, 1639–1642 (1999). https://doi.org/10.1038/sj.onc.1202461

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