Abstract
The function of the p53 tumor suppressor protein is regulated by interaction with Mdm2, which targets p53 for ubiquitin dependent degradation. We show here that like p53, p73α forms an interaction with Mdm2, both in vitro and in cells, but this does not result in the degradation of the p73α protein. The human papillomavirus E6 protein also fails to degrade p73α, suggesting that the mechanisms governing p73α stability are distinct from those known to regulate p53 stability. However, the interaction of Mdm2 with 73α is sufficient to impede p73α transcriptional function, despite the lack of degradation.
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Acknowledgements
We are very grateful to Bill Kaelin for the gift of the p73α expression construct. We also thank members of the Vousden Lab, for their support and encouragement with this work. This work was supported by the National Cancer Institute under contract with ABL.
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Bálint, E., Bates, S. & Vousden, K. Mdm2 binds p73α without targeting degradation. Oncogene 18, 3923–3929 (1999). https://doi.org/10.1038/sj.onc.1202781
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DOI: https://doi.org/10.1038/sj.onc.1202781
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