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  • Original Paper
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HuD, a neuronal-specific RNA-binding protein, is a putative regulator of N-myc pre-mRNA processing/stability in malignant human neuroblasts

Oncogene volume 18, pages 2703–2710 (1999)Cite this article

Abstract

N-myc gene copy numbers and transcription rates are similar in N (neuroblastic, tumorigenic) and S (non-neuronal, non-tumorigenic) neuroblastoma cells with chromosomally integrated amplified N-myc genes. However, N cells show significantly higher N-myc mRNA levels than S cells. Therefore, post-transcriptional control of N-myc gene expression must differ between these cell types. Since no differences in N-myc mRNA half-life were found between N and S cells from two cell lines, steady-state levels of N-myc pre-mRNA processing intermediates were analysed. Results suggest that the differences in N-myc expression arise primarily at the nuclear post-transcriptional level. The neuronal-specific RNA-binding Hu proteins are present in cytoplasmic and nuclear fractions of N cells and one of them, HuD, binds specifically to both exonic and intronic N-myc RNA sequences. In sense and antisense HuD-transfected N cells, there are coordinate changes in HuD and N-myc expression levels. Thus, we propose that HuD plays a role in the nuclear processing/stability of N-myc pre-mRNA in N-type neuroblastoma cells.

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Acknowledgements

We thank Drs M Bordonaro, GT Manley, S Nikolopoulos, JB Posner, M Rosenfeld, BY Rubin, JG Scammell and PS Smitt for providing materials used in this work. This research was supported by the GRATIS Fund.

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  1. Darina L Lazarova

    Present address: Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, 06520, Connecticut, USA

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  1. Department of Biological Sciences, Fordham University, 441 E Fordham Road, Bronx, 10458, New York, USA

    Darina L Lazarova, Barbara A Spengler, June L Biedler & Robert A Ross

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Lazarova, D., Spengler, B., Biedler, J. et al. HuD, a neuronal-specific RNA-binding protein, is a putative regulator of N-myc pre-mRNA processing/stability in malignant human neuroblasts. Oncogene 18, 2703–2710 (1999). https://doi.org/10.1038/sj.onc.1202621

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