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Unusual β-sheet periodicity in small cyclic peptides

Nature Structural Biology volume 5, pages 284–288 (1998)Cite this article

Abstract

Cyclic peptide homologs of gramicidin S containing 6, 8, 10, 12, 14 and 16 residues were synthesized and characterized using circular dichroism (CD) and 1H NMR spectroscopy. Based on the three-dimensional structures generated from these data we have found strong evidence of a periodic sequence-length dependence on β-sheet content. In particular, peptides of length 6, 10 and 14 residues exhibit a high β-sheet content, while peptides of 8, 12 and 16 residues appear to exist as random coils. This unusual β-sheet periodicity may have important implications in our understanding of β-sheet formation and in the design of constrained β-sheet and β-hairpin mimics.

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Authors and Affiliations

  1. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2N8, Canada

    Alan C. Gibbs, Alex M. Nip & David S. Wishart

  2. Protein Engineering Network of Centres of Excellence and the Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2N8, Canada

    Leslie H. Kondejewski, Wolfram Gronwald, Robert S. Hodges & Brian D. Sykes

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  1. Alan C. Gibbs
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  2. Leslie H. Kondejewski
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  3. Wolfram Gronwald
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  4. Alex M. Nip
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  5. Robert S. Hodges
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  7. David S. Wishart
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Correspondence to David S. Wishart.

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Gibbs, A., Kondejewski, L., Gronwald, W. et al. Unusual β-sheet periodicity in small cyclic peptides. Nat Struct Mol Biol 5, 284–288 (1998). https://doi.org/10.1038/nsb0498-284

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