1. Maria M. Konarska is at the Rockefeller University, New York, New York 10065, USA
   e-mail: konarsk@mail.rockefeller.edu

Abstract

The compositional complexity of the spliceosome creates a serious obstacle for its experimental analysis. Purification of a compositionally defined splicing complex C capable of completing the second step of splicing in the absence of additional proteins opens the door for future mechanistic and structural analyses.

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