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A purified catalytically competent spliceosome

Nature Structural & Molecular Biology volume 15, pages 222–224 (2008)Cite this article

The compositional complexity of the spliceosome creates a serious obstacle for its experimental analysis. Purification of a compositionally defined splicing complex C capable of completing the second step of splicing in the absence of additional proteins opens the door for future mechanistic and structural analyses.

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Figure 1: Schematic of selected transitions along spliceosome assembly, catalysis and disassembly.

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Acknowledgements

I thank D. Smith and C. Query for comments and discussions.

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Authors and Affiliations

  1. Maria M. Konarska is at the Rockefeller University, New York, New York 10065, USA konarsk@mail.rockefeller.edu,

    Maria M Konarska

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  1. Maria M Konarska
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Konarska, M. A purified catalytically competent spliceosome. Nat Struct Mol Biol 15, 222–224 (2008). https://doi.org/10.1038/nsmb0308-222

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