Access
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
Analysis
Nature Structural & Molecular Biology 15, 1223–1227 (1 November 2008) | doi:10.1038/nsmb.1501
The 'glutamate switch' provides a link between ATPase activity and ligand binding in AAA+ proteins
&
Abstract
AAA+ proteins carry out diverse functions in cells. In most cases, their ATPase activity is tightly regulated by protein partners and target ligands, but the mechanism for this control has remained unclear. We have identified a conserved link between the ligand binding and ATPase sites in AAA+ proteins. This link, which we call the 'glutamate switch', regulates ATPase activity directly in response to the binding of target ligands by controlling the orientation of the conserved glutamate residue in the DExx motif, switching it between active and inactive conformations. The reasons for this level of control of the ATPase activity are discussed in the context of the biological processes catalyzed by AAA+ proteins.
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
