Nature Structural & Molecular Biology
- 13, 950 - 951 (2006)
doi:10.1038/nsmb1106-950
RNA ligase does the AMP shuffleJohn M Pascal1 & Tom Ellenberger21
John M. Pascal is in the Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
2
Tom Ellenberger is in the Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA. tome@biochem.wustl.edu
Crystal structures of T4 RNA ligase 2 trapped at different stages of a multistep reaction show how one enzyme active site juggles three phosphoryl transfer reactions to join two RNA strands. The structures also show how the ligase enzyme selectively recognizes the sugar pucker of ribose to achieve catalytic specificity toward RNA-containing substrates.
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