Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Image shows LNCaP prostate cancer cells spiked into peripheral blood mononuclear cells, taken while developing an assay for detecting circulating tumour cells. The larger LNCaP cells show a punctate staining pattern following RNA in situ hybridization using a cocktail of fluorescent probes specific for cells of epithelial origin (EpCAM, KRT8, KRT18, and KRT19) and prostate cancer (PSA and PSMA). The smaller peripheral blood mononuclear cells show a circumferential staining pattern following incubation with a fluorescently labelled antibody against the leukocyte antigen CD45.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Image shows LNCaP prostate cancer cells spiked into peripheral blood mononuclear cells, taken while developing an assay for detecting circulating tumour cells. The larger LNCaP cells show a punctate staining pattern following RNA in situ hybridization using a cocktail of fluorescent probes specific for cells of epithelial origin (EpCAM, KRT8, KRT18, and KRT19) and prostate cancer (PSA and PSMA). The smaller peripheral blood mononuclear cells show a circumferential staining pattern following incubation with a fluorescently labelled antibody against the leukocyte antigen CD45.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Image shows LNCaP prostate cancer cells spiked into peripheral blood mononuclear cells, taken while developing an assay for detecting circulating tumour cells. The larger LNCaP cells show a punctate staining pattern following RNA in situ hybridization using a cocktail of fluorescent probes specific for cells of epithelial origin (EpCAM, KRT8, KRT18, and KRT19) and prostate cancer (PSA and PSMA). The smaller peripheral blood mononuclear cells show a circumferential staining pattern following incubation with a fluorescently labelled antibody against the leukocyte antigen CD45.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Image shows LNCaP prostate cancer cells spiked into peripheral blood mononuclear cells, taken while developing an assay for detecting circulating tumour cells. The larger LNCaP cells show a punctate staining pattern following RNA in situ hybridization using a cocktail of fluorescent probes specific for cells of epithelial origin (EpCAM, KRT8, KRT18, and KRT19) and prostate cancer (PSA and PSMA). The smaller peripheral blood mononuclear cells show a circumferential staining pattern following incubation with a fluorescently labelled antibody against the leukocyte antigen CD45.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Image shows LNCaP prostate cancer cells spiked into peripheral blood mononuclear cells, taken while developing an assay for detecting circulating tumour cells. The larger LNCaP cells show a punctate staining pattern following RNA in situ hybridization using a cocktail of fluorescent probes specific for cells of epithelial origin (EpCAM, KRT8, KRT18, and KRT19) and prostate cancer (PSA and PSMA). The smaller peripheral blood mononuclear cells show a circumferential staining pattern following incubation with a fluorescently labelled antibody against the leukocyte antigen CD45.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Cover image supplied by Michael A. Gorin, James E. Verdone and Kenneth J. Pienta, The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.