FIGURE 1 | From cell to tomogram.

From the following article:

Cryo-electron tomography of bacteria: progress, challenges and future prospects

Jacqueline L.S. Milne & Sriram Subramaniam

Nature Reviews Microbiology 7, 666-675 (September 2009)

doi:10.1038/nrmicro2183

Cryo-electron tomography of bacteria: progress, challenges and future prospects

Schematic diagram illustrating the sequence of steps involved in obtaining a three-dimensional image of a plunge-frozen cell using cryo-electron tomography. a | A small volume (typically approx 3–5 mul) of a suspension of cells in culture is deposited on a holey carbon grid and then plunge-frozen in liquid ethane cooled to temperatures of approx 100 K by liquid nitrogen. b | The grid is then transferred into the column of an electron microscope for collection of a series of images at varying tilts. c | The series of tilted images is then converted to a three-dimensional volume (tomogram) that provides a representation of the distribution of densities of cellular components.

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