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News and Views
Nature Photonics 2, 131 - 132 (2008)
doi:10.1038/nphoton.2008.09
Holography: Scan-free three-dimensional imaging
Ting-Chung Poon1
- Ting-Chung Poon is at the Bradley Department of Electrical and Computer Engineering at Virginia Tech, Virginia 24061, USA.
e-mail: tcpoon@vt.edu
Abstract
A holographic microscope that can capture fluorescent images of three-dimensional specimens without the need for axial scanning looks set to bring benefits to biomedical imaging.
Three-dimensional (3D) imaging is a formidable task in optical microscopy as it is well-known that the greater the lateral resolution of an image, the smaller the depth of field. In other words, a detailed high-resolution image can be obtained using a powerful high-numerical-aperture lens, such as a microscope objective, but the compromise is the ability to image only a thin section of the specimen.
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