Abstract
Early detection of the protein marker troponin I in patients with a higher risk of acute myocardial infarction1,2,3,4,5 can reduce the risk of death from heart attacks6,7,8,9,10. Most troponin assays are currently based on the conventional enzyme linked immunosorbent assay and have detection limits in the nano- and picomolar range11. Here, we show that by combining viral nanoparticles, which are engineered to have dual affinity for troponin antibodies and nickel, with three-dimensional nanostructures including nickel nanohairs, we can detect troponin levels in human serum samples that are six to seven orders of magnitude lower than those detectable using conventional enzyme linked immunosorbent assays11,12,13,14,15,16. The viral nanoparticle helps to orient the antibodies for maximum capture of the troponin markers. High densities of antibodies on the surfaces of the nanoparticles and nanohairs lead to greater binding of the troponin markers, which significantly enhances detection sensitivities. The nickel nanohairs are re-useable and can reproducibly differentiate healthy serum from unhealthy ones. We expect other viral nanoparticles to form similar highly sensitive diagnostic assays for a variety of other protein markers.
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Acknowledgements
This study was supported by the National Research Laboratory Project (a main project that supported this work) of the Ministry of Education, Science and Technology (grant no. ROA-2007-000-20084-0), the Korea Health 21 R&D Project of the Ministry of Health, Welfare and Family Affairs of the Republic of Korea (grant no. A050750), the Pioneer Research Center Program (grant no. M10711160001-08M1116-00110), the Microbial Genomics and Applications Center at KRIBB, the Seoul R&BD program (no. 10920), and the Korea Research Foundation (grant no. KRF-2006-005-J03603).
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J.L. conceived the viral particle-based assay experiments, conducted the data analysis and wrote the paper. Y.K.K. conceived the nanohair concept, conducted the data analysis and co-wrote the paper. J.S.P. and E.J.L. performed the viral particle synthesis and assay experiments and collected the data. M.K.C. performed the nanohair synthesis and SEM analysis. J.S.P., E.J.L. and M.K.C. contributed equally to this work. K.Y.A. analysed assay data. K.E.L. and S.-S.H. contributed TEM analysis tools. J.H.J. and Y.C. contributed human sera for diagnostic assay.
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Park, JS., Cho, M., Lee, E. et al. A highly sensitive and selective diagnostic assay based on virus nanoparticles. Nature Nanotech 4, 259–264 (2009). https://doi.org/10.1038/nnano.2009.38
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DOI: https://doi.org/10.1038/nnano.2009.38
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