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DART (drug acutely restricted by tethering) enables the manipulation of native receptors on genetically defined neurons. This work describes second-generation DART reagents for manipulating GABAA and AMPA receptors with higher cellular specificity than previously achieved.
Although not widely appreciated, measured smFRET efficiencies can vary depending on illumination intensity. Self-healing fluorophores offer robust triplet state suppression to enable accurate smFRET measurements under broad illumination regimes.
Tissues, organs and organ systems are composed of interacting cells (the cellome). We discuss the emergence of an omics approach that we refer to as cellomics. It enables cellome-wide analysis in whole-organ or whole-body specimens, based on advanced three-dimensional imaging and image analysis technology. We think that cellomics will pave the way for the incorporation of cellular, intercellular and spatial information across millions of cells in our body.
Blush regularization makes use of a neural network pre-trained on a diverse set of high-resolution cryo-EM half-maps to improve image alignment, effectively lowering the size barrier, during cryo-EM structure determination.
This Registered Report presents the results of the Long-read RNA-Seq Genome Annotation Assessment Project, which is a community effort for benchmarking long-read methods for transcriptome analyses, including transcript isoform detection, quantification and de novo transcript detection.
Deconwolf is a computationally efficient and user-friendly software tool for fluorescence microscopy image deconvolution that improves the analysis of diverse fluorescence in situ hybridization methods and can handle large datasets.
scFoundation, with 100 million parameters covering about 20,000 genes, pretrained on over 50 million single-cell transcriptomics profiles, is a foundation model for diverse tasks of single-cell analysis.
Integrative and reference-informed tissue segmentation (IRIS) harnesses single-cell RNA sequencing data for the accurate identification of spatial domains in spatially resolved transcriptomics. IRIS is computationally efficient and uniquely suited for analyzing large datasets.
A fundamental mechanism for information processing in the brain is electrical activity. However, observing such activity at the single-cell level is challenging. We have developed an optical microscope that combines the advantages of targeted illumination and confocal gating to enable kilohertz-rate voltage imaging across large fields of view in thick tissue.
In targeted-illumination confocal microscopy, the combination of targeted illumination with confocal detection allows one-photon voltage imaging across large fields of view with a high signal-to-noise ratio.
Photoconversion of popular organic dyes results in blue-shifted emission and altered fluorescence lifetimes that can cause artifacts in quantitative microscopy. These can be avoided through proper labeling strategies such as using exchangeable dyes.
Comparing brain connectivity between chimpanzees and humans is a means of understanding human cognition and evolution. To address the scarcity of chimpanzee neuroimaging data, we introduce a high-quality MRI resource that reveals previously unseen anatomical details, offering valuable insights into human brain evolution.