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Correspondence
Nature Methods 6, 477–478 (1 July 2009) | doi:10.1038/nmeth0709-477
Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates
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Abstract
To the Editor: Currently, the most widely used method for purifying recombinant proteins for biochemical and especially structural studies is immobilized metal affinity chromatography (IMAC), in which a metal-binding polyhistidine tag (His tag) serves as a small purification handle on the target protein. IMAC is a powerful and generic purification method, with high recovery yields and low costs.
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