This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
Nature Communications Open Access 18 May 2017
-
High-efficient production and biophysical characterisation of nicastrin and its interaction with APPC100
Scientific Reports Open Access 09 March 2017
-
A fast and easy strategy for protein purification using “teabags”
Scientific Reports Open Access 30 June 2016
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Chaga, G.S. J. Biochem. Biophys. Methods 49, 313–334 (2001).
Manjasetty, B.A., Turnbull, A., Panjikar, S., Büssow, K. & Chance, M.R. Proteomics 8, 612–625 (2008).
Peti, W. & Page, R. Protein Expr. Purif. 51, 1–10 (2007).
Structural Genomics Consortium et al. Nat. Methods 5, 135–146 (2008).
Neilands, J.B. J. Biol. Chem. 270, 26723–26726 (1995).
Acknowledgements
We thank S.-O. Enfors and D. Hart for valuable comments. The Structural Genomics Consortium is a registered charity (1097737) that receives funds from Sweden, UK and Canada (http://www.thesgc.com/about/sponsors.php/)
Author information
Authors and Affiliations
Corresponding author
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1-3 and Supplementary Methods (PDF 1017 kb)
Rights and permissions
About this article
Cite this article
Magnusdottir, A., Johansson, I., Dahlgren, LG. et al. Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates. Nat Methods 6, 477–478 (2009). https://doi.org/10.1038/nmeth0709-477
Issue Date:
DOI: https://doi.org/10.1038/nmeth0709-477
This article is cited by
-
Characterization of a Pinus sylvestris thaumatin-like protein gene and determination of antimicrobial activity of the in vitro expressed protein
Tree Genetics & Genomes (2018)
-
Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
Nature Communications (2017)
-
High-efficient production and biophysical characterisation of nicastrin and its interaction with APPC100
Scientific Reports (2017)
-
A fast and easy strategy for protein purification using “teabags”
Scientific Reports (2016)