Brief Communication abstract
Nature Methods 6, 500 - 502 (2009)
Published online: 31 May 2009 | doi:10.1038/nmeth.1337
Protein interaction platforms: visualization of interacting proteins in yeast
Alexa M Schmitz1, Monica F Morrison1, Akochi O Agunwamba1, Max L Nibert2 & Cammie F Lesser1,2
Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein 
NS, which forms large focal inclusions in living cells. When a query protein is fused to NS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.
- Department of Medicine (Microbiology and Molecular Genetics), Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Cambridge, Massachusetts, USA.
- Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, USA.
Correspondence to: Cammie F Lesser1,2 e-mail: clesser@partners.org
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