Brief Communication abstract
Nature Methods 6, 211 - 213 (2009)
Published online: 1 February 2009 | doi:10.1038/nmeth.1299
Programmed subcellular release for studying the dynamics of cell detachment
Bridget Wildt1, Denis Wirtz1,2,3 & Peter C Searson1,2,3
Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland 21218, USA.
- Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland 21218, USA.
- Howard Hughes Medical Institute Graduate Training Program and the Institute for NanoBioTechnology, Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland 21218, USA.
Correspondence to: Denis Wirtz1,2,3 e-mail: wirtz@jhu.edu
Correspondence to: Peter C Searson1,2,3 e-mail: searson@jhu.edu
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