Brief Communication abstract


Nature Methods 6, 813 - 816 (2009)
Published online: 11 October 2009 | doi:10.1038/nmeth.1389

A general life-death selection strategy for dissecting protein functions

Po Hien Ear1 & Stephen W Michnick1

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Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.

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  1. Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.

Correspondence to: Stephen W Michnick1 e-mail: stephen.michnick@umontreal.ca



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