Supplementary information

From the following article

Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution

Bo Huang, Sara A Jones, Boerries Brandenburg & Xiaowei Zhuang

Nature Methods 5, 1047 - 1052 (2008) Published online: 23 November 2008

doi:10.1038/nmeth.1274

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Supplementary Text and Figures

Supplementary Figures 1-7, Supplementary Data and Supplementary Methods

Supplementary Video 1

A 3D presentation of the mitochondria shown in Figure 2a. Different viewing perspectives of the 3D image are presented by rotating the image. In both x-y and x-z viewing perspectives, consecutive 100 nm thick sections are also shown. The x-y sections start from the bottom of the cells toward top and the x-z sections start from the upper part of the image. Scale bar: 3 mum.

Supplementary Video 2

A 3D presentation of the mitochondrion and microtubules shown in Figure 3e. Different viewing perspectives of the 3D STORM image (left) are presented by rotating the image. A 3D surface rendered model (right) is also shown to facilitate understanding the spatial relationship between the mitochondrion and microtubules. The model is created by manually tracing the six microtubules and the surface of the mitochondrion in each of the 20 nm thick z sections (30 sections in total), a method that is commonly used in modeling of electron microscopy tomography data. The individual surface segments were then 3D rendered by using the ImageSurfer software (http://www.imagesurfer.org).

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