Nature Methods
- 4, 223 - 225 (2007)
Published online: 25 February 2007; | doi:10.1038/nmeth1013
Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detectionChristopher Deufel1, Scott Forth1, Chad R Simmons1, 2, Siavash Dejgosha1 & Michelle D Wang11
Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA. 2
Present address: Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.
Correspondence should be addressed to Michelle D Wang mwang@physics.cornell.edu We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.
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