Nature Methods
- 4, 127 - 129 (2007)
Published online: 28 January 2007; | doi:10.1038/nmeth1009
Improved calcium imaging in transgenic mice expressing a troponin C–based biosensorNicola Heim1, 3, Olga Garaschuk2, 3, Michael W Friedrich1, Marco Mank1, Ruxandra I Milos2, Yury Kovalchuk2, Arthur Konnerth2 & Oliver Griesbeck11
Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 Martinsried, Germany. 2
Institute of Neuroscience, Technical University Munich, Biedersteinerstr. 29, 80802 Munich, Germany. 3
These authors contributed equally to this work.
Correspondence should be addressed to Oliver Griesbeck griesbeck@neuro.mpg.de or Arthur Konnerth arthur.konnerth@lrz.tu-muenchen.de Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
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