Nature Methods
- 4, 1011 - 1013 (2007)
Published online: 4 November 2007; | doi:10.1038/nmeth1127
Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimeraIan Burbulis, Kumiko Yamaguchi, Richard Yu, Orna Resnekov & Roger Brent
Center for Quantitative Genome Function and Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA.
Correspondence should be addressed to Roger Brent brent@molsci.org We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.
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