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Directed evolution by in vitro compartmentalization

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Figure 1: Schematic representation of directed evolution by IVC.
Figure 2: A w/o/w emulsion generated by extrusion.
Figure 3: Droplet size in a w/o emulsion versus homogenization speed and the relationship with the activity of in vitro expressed enzymes.

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Correspondence to Dan S Tawfik or Andrew D Griffiths.

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The Medical Research Council (MRC) and the Weizmann Institute have filed patent applications, which include some of the ideas described in this manuscript. Should these organizations receive revenues from licensing these patents, the authors may be entitled to receive payments through the organizations’ Rewards to Inventors schemes.

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Miller, O., Bernath, K., Agresti, J. et al. Directed evolution by in vitro compartmentalization. Nat Methods 3, 561–570 (2006). https://doi.org/10.1038/nmeth897

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