Volume 12 Issue 8, August 2015

How to use diamonds to image single cells, and how to have a career without ever applying for a job.

Fibroblasts pass through a pluripotent state when undergoing transdifferentiation in the presence of pluripotency factors.

Injection of a flexible mesh containing electronics enables neural recordings in the mouse brain in a minimally invasive fashion.

An optimized strategy allows genetic encoding of phosphoserine and its nonhydrolyzable analog.

Researchers design programmable two-dimensional (2D) protein lattices that may find broad applications in diverse fields.

Cas9 proteins with altered PAM specificities widen the target range of the CRISPR system.

Large-scale phenotyping is generating much data that geneticists can harness. Amid the excitement about the possibilities, there are some points of caution.

An integrated single-molecule fluorescence approach enables the study of nano-to-millisecond protein conformational dynamics in living mammalian cells.

This Analysis reports a comparison of current software packages for single-molecule localization in localization-based super-resolution imaging. Performance of the participating software on synthetic, biologically inspired ground-truth data was assessed by multiple criteria.

A quantitative mass spectrometry–based standard operating procedure to classify antibody performance in immunoprecipitation is assessed in a multilaboratory study.

By error-correcting long nanopore reads and calling a consensus sequence using nanopore signal data, an entire bacterial genome is assembled de novo.

This paper reports magnetic imaging of immunolabeled mammalian cells using nitrogen-vacancy centers in diamond and shows that the method can be used for quantitative profiling of markers.

The combination of immunofluorescence and single-molecule FISH (smFISH) enables a quantitative description of how transcription factor binding drives the kinetics of mRNA production.

Image reconstruction by integrating exchangeable single-molecule localization (IRIS) allows for super-resolution imaging of multiple targets. IRIS yields continuously labeled structures owing to high labeling density and is demonstrated for multiple cytoskeletal components.

An NMR chemical shift–guided protein alignment method is combined with a comparative modeling pipeline to readily solve structures of large proteins.

A hybrid method that combines sparse NMR spectroscopy data with evolutionary residue-residue coupling information is used to solve accurate structures of large proteins.

The multi-trait set test (mtSet) is an efficient mixed-model method that enables genome-wide association tests for large cohorts and multiple quantitative traits using sets of variants to improve power and sensitivity, while addressing confounding due to population structure.

An optical phase-locked ultrasound lens integrated into a two-photon microscope enables continuous volumetric imaging of biological processes in vivo.

An infrared fluorescent protein based on a new monomeric scaffold is described in this paper and is tested for protein fusion in cells and in vivo.

Unique mutational signatures induced by cross-linking of m6A-specific antibodies to RNA identify m6A and m6Am residues at single-nucleotide resolution, transcriptome-wide.

Individual protein dynamics can be studied in live eukaryotic cells from the millisecond to nanosecond level using an integrated approach to confocal single-molecule FRET spectroscopy.

A combination of single-molecule long-read sequencing, single-molecule genome mapping and short-read sequencing provides reference-quality de novo assemblies and also shows improved phasing and variant detection over short-read assemblies when mapping to a reference genome.

This paper reports an approach to measure equilibrium binding affinities for interacting proteins in high throughput, allowing the rapid and quantitative profiling of the specificity of interaction motifs.