Abstract
To take advantage of the potential quantitative benefits offered by tandem mass spectrometry, we have modified the method in which tandem mass spectrum data are acquired in 'shotgun' proteomic analyses. The proposed method is not data dependent and is based on the sequential isolation and fragmentation of precursor windows (of 10 m/z) within the ion trap until a desired mass range has been covered. We compared the quantitative figures of merit for this method to those for existing strategies by performing an analysis of the soluble fraction of whole-cell lysates from yeast metabolically labeled in vivo with 15N. To automate this analysis, we modified software (RelEx) previously written in the Yates lab to generate chromatograms directly from tandem mass spectra. These chromatograms showed improvements in signal-to-noise ratio of approximately three- to fivefold over corresponding chromatograms generated from mass spectrometry scans. In addition, to demonstrate the utility of the data-independent acquisition strategy coupled with chromatogram reconstruction from tandem mass spectra, we measured protein expression levels in two developmental stages of Caenorhabditis elegans.
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Acknowledgements
The authors would especially like to thank M. MacCoss for his insightful comments about the manuscript and detailed knowledge of RelEx. They thank H. McDonald and C. Wu for discussion and critical reading of the manuscript, and H.R. Horvitz, A.P. Page and J. Ahnn for the SQV-4, CYP-5, and CRT-1 antibodies. J.D.V. and J.W. are supported by National Research Service Award (NIH) fellowships. J.R.Y. is supported by National Institutes of Health grants RR11823, R01MH067880 and R21067598-01.
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Supplementary information
Supplementary Fig. 1
Example tandem mass spectra obtained using different isolation widths (a) 1, (b) 20, and (c) 100 m/z for a 1 pmol/μl infusion of Glu-fibrinopeptide B. (PDF 46 kb)
Supplementary Fig. 2
Relex screenshots detailing the 6 reconstructed chromatograms from MS scans used to quantify RPL19A in the 1:1 yeast whole cell lysate standard mixture. (PDF 76 kb)
Supplementary Fig. 3
Relex screenshots detailing the 6 reconstructed chromatograms from MS scans used to quantify RPL19A in the 10:1 yeast whole cell lysate standard mixture. (PDF 73 kb)
Supplementary Fig. 4
Relex screenshots detailing the 10 reconstructed chromatograms from tandem mass spectra used to quantify RPL19A in the 1:1 yeast whole cell lysate standard mixture. (PDF 95 kb)
Supplementary Fig. 5
Relex screenshots detailing the 7 reconstructed chromatograms from tandem mass spectra used to quantify RPL19A in the 10:1 yeast whole cell lysate standard mixture. (PDF 60 kb)
Supplementary Table 1
Signal to noise enhancements we observed for chromatograms extracted from both MS scans and tandem mass spectra for a collection of peptides identified in a whole cell yeast lysate. (PDF 17 kb)
Supplementary Table 2
Averages and standard deviations of protein ratios identified from unlabeled and metabolically 15N labeled peptides from whole cell yeast lysates mixed in known ratios (PDF 19 kb)
Supplementary Table 3
Relex output file showing the normalized, average ion current ratios for 573 proteins identified and quantified from tandem mass spectra. Individual peptide ratio measurements are shown as well. (PDF 289 kb)
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Venable, J., Dong, MQ., Wohlschlegel, J. et al. Automated approach for quantitative analysis of complex peptide mixtures from tandem mass spectra. Nat Methods 1, 39–45 (2004). https://doi.org/10.1038/nmeth705
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DOI: https://doi.org/10.1038/nmeth705
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