Image: Laura Persat, Lausanne, Switzerland. Cover design: Erin Dewalt

Read the February issue

Latest Research

  • Brief Communication |

    Engineered nanobody allows reversible control of activity in cells through the binding of small molecules.

    • Helen Farrants
    • , Miroslaw Tarnawski
    • , Thorsten G. Müller
    • , Shotaro Otsuka
    • , Julien Hiblot
    • , Birgit Koch
    • , Moritz Kueblbeck
    • , Hans-Georg Kräusslich
    • , Jan Ellenberg
    •  & Kai Johnsson
  • Article |

    Mass cytometry in combination with a thiol-reactive barcoding strategy allows analysis and comparison of cell-type-specific signaling networks in organoids.

    • Xiao Qin
    • , Jahangir Sufi
    • , Petra Vlckova
    • , Pelagia Kyriakidou
    • , Sophie E. Acton
    • , Vivian S. W. Li
    • , Mark Nitz
    •  & Christopher J. Tape
  • Article |

    SMAC-seq combines long-read sequencing with open chromatin methylation by DNA methyltransferases to enable mapping of nucleosome position and chromatin accessibility.

    • Zohar Shipony
    • , Georgi K. Marinov
    • , Matthew P. Swaffer
    • , Nicholas A. Sinnott-Armstrong
    • , Jan M. Skotheim
    • , Anshul Kundaje
    •  & William J. Greenleaf
  • Brief Communication |

    Reverberation two-photon microscopy enables video-rate multiplane neuroimaging by performing near-instantaneous axial scanning over large depth ranges while maintaining 3D micrometer-scale resolution.

    • Devin R. Beaulieu
    • , Ian G. Davison
    • , Kıvılcım Kılıç
    • , Thomas G. Bifano
    •  & Jerome Mertz

News & Comment

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.