Mid-infrared metabolic imaging with vibrational probes

Latest Research

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    The Ribosolve pipeline combines single-particle cryo-EM, M2-seq biochemical analysis and Rosetta auto-DRRAFTER modeling to guide three-dimensional RNA structure determination.

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    PULSE is an optogenetic tool that consists of two modules with different wavelength sensitivities. Their interplay enables optogenetic access to gene expression in plants independently of ambient light.

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  • Brief Communication |

    Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.

    • Sebastian Strauss
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  • Article |

    Small vibrational tags (azide, 13C-edited carbonyl and deuterium-labeled probes) were introduced as metabolic probes for mid-infrared imaging. The tags allow unprecedented in situ visualization of metabolism in cells and animals with high information throughput.

    • Lixue Shi
    • , Xinwen Liu
    • , Lingyan Shi
    • , H. Ted Stinson
    • , Jeremy Rowlette
    • , Lisa J. Kahl
    • , Christopher R. Evans
    • , Chaogu Zheng
    • , Lars E. P. Dietrich
    •  & Wei Min
  • Brief Communication |

    CARPID uses CRISPR technology to navigate biotin ligase to specific lncRNAs, which allows proximal labeling and thus the querying of RNA–protein interactions in living cells.

    • Wenkai Yi
    • , Jingyu Li
    • , Xiaoxuan Zhu
    • , Xi Wang
    • , Ligang Fan
    • , Wenju Sun
    • , Linbu Liao
    • , Jilin Zhang
    • , Xiaoyu Li
    • , Jing Ye
    • , Fulin Chen
    • , Jussi Taipale
    • , Kui Ming Chan
    • , Liang Zhang
    •  & Jian Yan
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    Multifocal flat illumination for field-independent imaging (mfFIFI) enables patterned illumination over an extended field of view. Integration with instant structured illumination microscope allowed for high-speed, multicolor, volumetric super-resolution imaging over 100 × 100 µm2.

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    • , Kyle M. Douglass
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    • , Khalid A. Ibrahim
    • , Maeva Le Guennec
    • , Pierre Gönczy
    • , Virginie Hamel
    • , Paul Guichard
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News & Comment

  • News & Views |

    Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

    • Jane S. Richardson
  • Editorial |

    Systemic racism has held back under-represented minorities, especially Black people, in scientific research. We’re committed in our roles as journal editors to help correct this inequality.

  • Research Highlight |

    In situ labeling with azido sugars enables in vivo immunomodulation of dendritic cells.

    • Madhura Mukhopadhyay

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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