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Article
Nature Medicine 6, 278 - 282 (2000)
doi:10.1038/73128
Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells
Vijayakumar K. Ramiya1, Michael Maraist1, Karl E. Arfors2, Desmond A. Schatz3, Ammon B. Peck1,4 & Janet G. Cornelius4
Abstract
Ductal structures of the adult pancreas contain stem cells that differentiate
into islets of Langerhans. Here, we grew pancreatic ductal epithelial cells
isolated from prediabetic adult non-obese diabetic mice in long-term cultures,
where they were induced to produce functioning islets containing 
, 
and 
cells. These in vitro-generated islets showed temporal
changes in mRNA transcripts for islet cell-associated differentiation markers,
responded in vitro to glucose challenge, and reversed insulin-dependent
diabetes after being implanted into diabetic non-obese diabetic mice. The
ability to control growth and differentiation of islet stem cells provides
an abundant islet source for -cell reconstitution in type I diabetes.
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