Article abstract
Nature Medicine 13, 348 - 353 (2007)
Published online: 4 March 2007 | doi:10.1038/nm1547
ASC-J9 ameliorates spinal and bulbar muscular atrophy phenotype via degradation of androgen receptor
Zhiming Yang1,2,7, Yu-Jia Chang1,3,7, I-Chen Yu1, Shuyuan Yeh1, Cheng-Chia Wu1,3, Hiroshi Miyamoto1, Diane E Merry4, Gen Sobue5, Lu-Min Chen1,6, Shu-Shi Chang1,6 & Chawnshang Chang1
Abstract
Motor neuron degeneration resulting from the aggregation of the androgen receptor with an expanded polyglutamine tract (AR-polyQ) has been linked to the development of spinal and bulbar muscular atrophy (SBMA or Kennedy disease). Here we report that adding 5-hydroxy-1,7-bis(3,4-dimethoxyphenyl)-1,4,6-heptatrien-3-one (ASC-J9) disrupts the interaction between AR and its coregulators, and also increases cell survival by decreasing AR-polyQ nuclear aggregation and increasing AR-polyQ degradation in cultured cells. Intraperitoneal injection of ASC-J9 into AR-polyQ transgenic SBMA mice markedly improved disease symptoms, as seen by a reduction in muscular atrophy. Notably, unlike previous approaches in which surgical or chemical castration was used to reduce SBMA symptoms, ASC-J9 treatment ameliorated SBMA symptoms by decreasing AR-97Q aggregation and increasing VEGF164 expression with little change of serum testosterone. Moreover, mice treated with ASC-J9 retained normal sexual function and fertility. Collectively, our results point to a better therapeutic and preventative approach to treating SBMA, by disrupting the interaction between AR and AR coregulators.
- George Whipple Lab for Cancer Research, Departments of Pathology, Urology, and Radiation Oncology, and The Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA.
- Zhejiang University and 2nd Hospital, Hangzhou 310009, China.
- Taipei Medical University and Hospital, Taipei 110, Taiwan.
- Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
- Nagoya University, Nagoya 466-8550, Japan.
- China Medical University and Hospital, Taichung 404, Taiwan.
- These authors contributed equally to this work.
Correspondence to: Chawnshang Chang1 e-mail: chang@URMC.rochester.edu
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