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Letter
Nature Medicine  11, 786 - 790 (2005)
Published online: 5 June 2005; | doi:10.1038/nm1258

Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses

Steven M Jones1, 2, 9, Heinz Feldmann1, 3, 9, Ute Ströher1, 3, Joan B Geisbert4, Lisa Fernando1, Allen Grolla1, Hans-Dieter Klenk5, Nancy J Sullivan6, Viktor E Volchkov7, Elizabeth A Fritz4, Kathleen M Daddario8, Lisa E Hensley4, Peter B Jahrling4 & Thomas W Geisbert4

1  Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.

2  Department of Immunology, University of Manitoba, Winnipeg, Manitoba R3E 3R2, Canada.

3  Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3E 3R2, Canada.

4  United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Maryland 21702, USA.

5  Institute of Virology, Philipps-University, Robert Koch Str. 17, 35037, Marburg, Germany.

6  Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Covent Drive, Bethesda, Maryland, USA.

7  Filovirus Laboratory, University Claude Bernard Lyon-1, INSERM U412, 69007 Lyon, France.

8  Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814, USA.

9  These authors contributed equally to this work.

Correspondence should be addressed to Heinz Feldmann Heinz_Feldmann@hc-sc.gc.ca
Vaccines and therapies are urgently needed to address public health needs stemming from emerging pathogens and biological threat agents such as the filoviruses Ebola virus (EBOV) and Marburg virus (MARV). Here, we developed replication-competent vaccines against EBOV and MARV based on attenuated recombinant vesicular stomatitis virus vectors expressing either the EBOV glycoprotein or MARV glycoprotein. A single intramuscular injection of the EBOV or MARV vaccine elicited completely protective immune responses in nonhuman primates against lethal EBOV or MARV challenges. Notably, vaccine vector shedding was not detectable in the monkeys and none of the animals developed fever or other symptoms of illness associated with vaccination. The EBOV vaccine induced humoral and apparent cellular immune responses in all vaccinated monkeys, whereas the MARV vaccine induced a stronger humoral than cellular immune response. No evidence of EBOV or MARV replication was detected in any of the protected animals after challenge. Our data suggest that these vaccine candidates are safe and highly efficacious in a relevant animal model.


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Nature Medicine
ISSN: 1078-8956
EISSN: 1546-170X
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