Nature Genetics
6, 236 - 244 (1994)
doi:10.1038/ng0394-236
Purification of CpG islands using a methylated DNA binding columnSally H. Cross1, Jillian A. Charlton1, Xinsheng Nan1
& Adrian P. Bird1
1Institute of Cell and Molecular Biology University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, UK CpG islands are short stretches of DNA containing a high density of non−methylated CpG dinucleotides, predominantly associated with coding regions. We have constructed an affinity matrix that contains the methyl−CpG binding domain from the rat chromosomal protein MeCP2, attached to a solid support. A column containing the matrix fractionates DNA according to its degree of CpG methylation, strongly retaining those sequences that are highly methylated. Using this column, we have developed a procedure for bulk isolation of CpG islands from human genomic DNA. As CpG islands overlap with approximately 60% of human genes, the resulting CpG island library can be used to isolate full−length cDNAs and to place genes on genomic maps.
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