Brief Communication abstract
Nature Genetics 40, 1413 - 1415 (2008)
Published online: 2 November 2008 | Corrected online: 28 April 2009 | doi:10.1038/ng.259
There is an Addendum (June 2009) associated with this Brief Communication.
Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing
Qun Pan1, Ofer Shai1,2, Leo J Lee1,2, Brendan J Frey1,2 & Benjamin J Blencowe1,3
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in 
20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from 95% of multiexon genes undergo alternative splicing and that there are 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.
- Banting and Best Department of Medical Research, University of Toronto, Toronto M5S 3E1, Canada.
- Department of Electrical and Computer Engineering, University of Toronto, Toronto M5S 3G4, Canada.
- Department of Molecular Genetics, University of Toronto, Toronto M5S 3E1, Canada.
Correspondence to: Benjamin J Blencowe1,3 e-mail: b.blencowe@utoronto.ca
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Molecular biology Power sequencingNature News and Views (26 Jun 2008)
The beginning of the end for microarrays?Nature Methods News and Views (01 Jul 2008)
See all 3 matches for News And ViewsRESEARCH
Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3BOncogene Original Article
Met-driven invasive growth involves transcriptional regulation of Arhgap12Oncogene Original Article
See all 49 matches for Research
