Article abstract


Nature Genetics 40, 1300 - 1306 (2008)
Published online: 19 October 2008 | doi:10.1038/ng.235

Structure and function of a transcriptional network activated by the MAPK Hog1

Andrew P Capaldi1, Tommy Kaplan2,3, Ying Liu1, Naomi Habib2,3, Aviv Regev4, Nir Friedman2 & Erin K O'Shea1


Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.

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  1. Howard Hughes Medical Institute, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Departments of Molecular and Cellular Biology and Chemistry and Chemical Biology, Northwest Labs, 52 Oxford Street, Cambridge, Massachusetts 02138, USA.
  2. School of Computer Science and Engineering, The Hebrew University, Jerusalem 91904, Israel.
  3. Department of Molecular Genetics and Biotechnology, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel.
  4. Department of Biology, Massachusetts Institute of Technology and The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Correspondence to: Erin K O'Shea1 e-mail: erin_oshea@harvard.edu



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